Production of high-quality SARS-CoV-2 antigens: impact of bioprocess and storage on glycosylation, biophysical attributes, and ELISA serologic tests performance

June 23, 2021 Taylor 0 Comments

Serological assays are helpful instruments to check SARS-CoV-2 unfold and, importantly, to determine people that have been already contaminated and could be probably resistant to a virus re-infection. SARS-CoV-2 Spike protein and its Receptor Binding Area (RBD) are the antigens with larger potential to develop SARS-CoV-2 serological assays. Furthermore, structural research of those antigens are key to know the molecular foundation for Spike interplay with angiotensin changing enzyme 2 receptor, hopefully enabling the event of COVID-19 therapeutics.

Thus, it’s pressing that vital quantities of this protein grew to become accessible on the highest high quality. On this work, we produced Spike and RBD in two human derived cell hosts: HEK293-E6 and Expi293F™. We evaluated the affect of various and scalable bioprocessing approaches on Spike and RBD manufacturing yields and, extra importantly, on these antigens’ high quality attributes. Utilizing destructive and optimistic sera collected from human donors, we present a wonderful efficiency of the produced antigens, assessed in serologic ELISA exams, as denoted by the excessive specificity and sensitivity of the check.

We present sturdy Spike productions with last yields of approx. 2 mg per liter of tradition that have been maintained independently of the manufacturing scale or cell tradition technique. To the most effective of our data, the ultimate yield of 90 mg per liter of tradition obtained for RBD manufacturing, was the best reported so far. An in-depth characterization of SARS-CoV-2 Spike and RBD proteins was carried out, specifically the antigen’s oligomeric state, glycosylation profiles and thermal stability throughout storage.

The correlation of those high quality attributes with ELISA efficiency present equal reactivity to SARS-CoV-2 optimistic serum, for all Spike and RBD produced, and for all storage circumstances examined. Total, we offer simple protocols to provide high-quality SARS-CoV-2 Spike and RBD antigens, that may be simply tailored to each tutorial and industrial settings; and combine, for the primary time, research on the affect of bioprocess with an in-depth characterization of those proteins, correlating antigen’s glycosylation and biophysical attributes to efficiency of COVID-19 serologic exams.

Analysis of IgM LAT and IgM ELISA as in comparison with microscopic agglutination check (MAT) for early analysis of Leptospira sp

Leptospirosis is a worldwide zoonotic illness attributable to spirochetes of the genus Leptospira. The scientific manifestation of leptospirosis is non-specific and continuously misdiagnosed as different sicknesses. The purpose of this research was to match the diagnostic accuracies of two industrial exams for early analysis of Leptospira species: the IgM latex agglutination check (IgM LAT) and the IgM enzyme-linked immunosorbent assay (IgM ELISA).

A complete of 140 serum samples have been obtained from sufferers suspected of leptospirosis on the Universiti Kebangsaan Malaysia Medical Centre (UKMMC). These serum samples have been examined for the presence of Leptospira sp. utilizing IgM LAT, IgM ELISA and MAT. From Desk 1, IgM LAT confirmed 21% (n = 29) optimistic, 18% (n = 25) inconclusive and 61% (n = 86) destructive, whereas IgM ELISA confirmed 6% (n = 8) optimistic, 6% (n = 8) inconclusive, 88% (n = 124) destructive and MAT confirmed 11% (n = 16) optimistic, 47% (n = 65) inconclusive, 42% (n = 59) destructive.

The sensitivity, specificity, optimistic predictive worth (PPV) and destructive predictive worth (NPV) of IgM LAT have been 68.8%, 57.6%, 30.6% and 87.2% respectively, whereas for IgM ELISA they have been 37.5%, 89.8%, 50% and 84.1%, respectively as in comparison with MAT (Desk 2). The outcomes confirmed that IgM LAT had larger sensitivity however decrease specificity in comparison with IgM ELISA.

In conclusion, IgM LAT could be helpful as an early screening check for early analysis of Leptospira sp., whereas IgM ELISA is an acceptable methodology for decreasing false destructive detection of Leptospira sp. As each exams present reasonable percentages (~65%) in accuracy, a further check is required for higher detection of Leptospira sp.

Seroprevalence of Toxoplasma gondii in ruminants through the use of latex agglutination check (LAT) and enzyme-linked immunosorbent assay (ELISA) in Assiut governorate

Toxoplasmosis is a crucial zoonotic parasitic illness world-wide. Nonetheless, knowledge on Toxoplasma gondii an infection in ruminants are scarce in Assiut governorate, Egypt. Thus we carried out a survey on the seroprevalence of T. gondii an infection through the use of latex agglutination check (LAT) and enzyme-linked immunosorbent assay (ELISA). A complete of 274 sera have been examined (56 imported camels, 56 cattle, 55 buffaloes, 50 sheep and 57 goats) within the interval from November 2014 – February 2016.

The general seroprevalence in ruminants was (35.8%) by LAT and (83.6%) by ELISA. The very best charge of T. gondii an infection by LAT was present in goats (47.4%) adopted by sheep (44%), camels (35.7%), cattle (32.1%) and buffaloes (20%). The very best charge of T. gondii an infection by ELISA was recorded in camels (96.4%) adopted by goats (87.7%), sheep (86%), buffaloes (74.5%) and cattle (73.2%). Antibody titres to T. gondii in optimistic sera by LAT ranged from 1:2 to 1:64 in imported camels, 1:2 to 1:32 in cattle, 1:2 to 1:Eight in buffaloes, from 1:Four to 1:64 in sheep and from 1:2 to 1:16 in goats. The variations of seroprevalence between completely different species have been vital by LAT and extremely vital by ELISA. Our outcomes counsel that the T. gondii parasite is extensively unfold on account of excessive publicity to infective cat faeces.

Serological Check to Decide Publicity to SARS-CoV-2: ELISA Based mostly on the Receptor-Binding Area of the Spike Protein (S-RBD _N318-V510) Expressed in Escherichia coli

Huge worldwide serological testing for SARS-CoV-2 is required to find out the extent of virus publicity in a selected area, the ratio of symptomatic to asymptomatic contaminated individuals, and the period and extent of immunity after an infection. To realize this, the event and manufacturing of dependable and cost-effective SARS-CoV-2 antigens is vital. We report the bacterial manufacturing of the peptide S-RBD_N318-V510, which incorporates the receptor-binding area of the SARS-CoV-2 spike protein (area of 193 amino acid residues from asparagine-318 to valine-510) of the SARS-CoV-2 spike protein.

We purified this peptide utilizing an easy method involving bacterial lysis, his-tag-mediated affinity chromatography, and imidazole-assisted refolding. The antigen performances of S-RBD_N318-V510 and a industrial full-length spike protein have been in contrast in ELISAs. In direct ELISAs, the place the antigen was instantly sure to the ELISA floor, each antigens discriminated sera from non-exposed and uncovered people. Nonetheless, the discriminating decision was higher in ELISAs that used the full-spike antigen than the S-RBD_N318-V510.

AssayStar 384 Square Well Assay Plate, Black Solid Bottom - 100 plates
WB2-4250	Westburg	each	Ask for price

96-Well Filter Plate
FCM-B01M	ACROBIOSYSTEMS	1plate	EUR 21.8

Description: The membrane of this plate has a consistent physical structure with a smooth surface morphology, this makes it ideal for use in bead based assays as the microspheres do not get trapped in the membrane, allowing for efficient bead recovery. The filter plate is suggested no more than 350μL and no less than 1.2μm loading.

96 or 384 well plate
HB-E130	Phoenix instrument	each	EUR 125

96 well filter plate (960ul each well)
SD5006	Bio Basic	12UNIT	EUR 115.85

Black 96 Well Plate, 1EA
X025-1EA	Arbor Assays	1EA	EUR 19

NanoECM®; 96 Well Plate
9601	Neuromics	each	EUR 38.7

Adapter for 96 well plate
3510147	Atto	1unit	EUR 4422

NanoAligned®; 96 Well Plate
9602	Neuromics	each	EUR 41.4

Aligned Nanofibers 96 Well Plate
3D00012-96	Neuromics	700 nm-PCLs	EUR 132

Streptavidin coated 96-well Plate
6523-5	Biovision	each	EUR 392.4

Deep Well Plate 96 Well 2.0 ML - PK50
AXYPDW20C	Scientific Laboratory Supplies	PK50	EUR 221.4

96 Deep Well Plate Square Well V - PK50
PCR0246	Scientific Laboratory Supplies	PK50	EUR 363.15

96 Well ELISA Plate, 8-Well, Detachable, High Binding, WhiteFrame & Black Well,
504261	NEST Biotechnology	50 pcs/cs	EUR 26.29
Description: 96 Well ELISA Plate, 8-Well, Detachable, High Binding, WhiteFrame & Black Well, 5/pk, 50/cs

UNIFILTER PLATE 96 WELL 800 UL/WELL - PK25
WHA77002803	Scientific Laboratory Supplies	PK25	EUR 1101.6

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY
P-DW-12-HC	CORNING	5/pk	EUR 182.4
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Cell Culture Plate, 96 Well
CCP01096-100Case	Vazyme	100 / Case	EUR 154.24

96 WELL PCR SEGMENTED PLATE
PCR-96-SG-C	CORNING	10/pk	EUR 174
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Tissue Culture Plate, 96 Well
TCP20-96	Bio Basic	1 UNIT	EUR 64.18

Brand 96 Well Deep Well Plate-Stackable - PK24
BR701354-24EA	Scientific Laboratory Supplies	PK24	EUR 183.6

96-Well EIA Microtiter Plate
MBS258056-1Plate	MyBiosource	1Plate	EUR 115

96-Well EIA Microtiter Plate
MBS258056-5x1Plate	MyBiosource	5x1Plate	EUR 335

Clear Coated 96 Well Plate, 1EA
C107-1EA	Arbor Assays	1EA	EUR 162

96-Well Protein Folding Plate
PFS096	ProFoldin	kit	EUR 365.23
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins.

96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & CLear Well,
504201	NEST Biotechnology	50 pcs/cs	EUR 38.5
Description: 96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & CLear Well, 5/pk, 50/cs

96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & White Well,
504271	NEST Biotechnology	50 pcs/cs	EUR 51.15
Description: 96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & White Well, 5/pk, 50/cs

96-well Black Polystyrene Plate
MBS258058-1Plate	MyBiosource	1Plate	EUR 115

96-well Black Polystyrene Plate
MBS258058-5x1Plate	MyBiosource	5x1Plate	EUR 340

BioMag® 96-Well Plate Separator
8MB4109S-1	Polysciences Europe GmbH	1magnet	EUR 1094.04

DEEP WELL PLATE 96 WELL 2.0 ML STERIL - PK50
AXYPDW20CS	Scientific Laboratory Supplies	PK50	EUR 270

96 well PCR plate ns - PK50
AXY2164	Scientific Laboratory Supplies	PK50	EUR 268.65

Polystyrene-bottomed 96-well plate + lid (ELISPOT)
CT365	U-CyTech	5	EUR 93.6

2 x Clear 96 well Plate, 2EA
X003-2EA	Arbor Assays	2EA	EUR 13

5 x Clear 96 well Plate, 5EA
X003-5EA	Arbor Assays	5EA	EUR 25

2 x Clear 96 well Plate, 2EA
X018-2EA	Arbor Assays	2EA	EUR 13

4 x Clear 96 Well Plate, 4EA
X018-4EA	Arbor Assays	4EA	EUR 31

96 Deep Well Plate Round Wells - PK50
PCR0242	Scientific Laboratory Supplies	PK50	EUR 329.4

Plate 96 Well Immulon II - PK50
3455	Scientific Laboratory Supplies	PK50	EUR 318.6

96 Well Roche Style PCR Plate
PCR1218	Scientific Laboratory Supplies	PK50	EUR 223.74

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTA55-5P	Alpha Diagnostics	1 PK	EUR 315.6

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTG65-5P	Alpha Diagnostics	1 PK	EUR 315.6

96 Deep Well Plate Square WellU - PK50
PCR0256	Scientific Laboratory Supplies	PK50	EUR 261.9

96 Well Skirted PCR Plate - PK50
PCR1236	Scientific Laboratory Supplies	PK50	EUR 433.35

96 well filter plate (no filter)
SD5006-EMPTY	Bio Basic	12UNIT	EUR 104.89

Nunc Lid for 96 well plate - PK50
264122	Scientific Laboratory Supplies	PK50	EUR 143.1

96 Deep Well Plate Square Wells - PK100
PCR0240	Scientific Laboratory Supplies	PK100	EUR 378

96 Well Semi Skirted Plate - PK50
PCR1216	Scientific Laboratory Supplies	PK50	EUR 229.5

Black Half Area 96 Well Plate, 1EA
X023-1EA	Arbor Assays	1EA	EUR 25

96 well DNA plate with membrane (960ul each well)
SD5007	Bio Basic	12UNIT	EUR 291.24

96 well RNA plate with membrane (960ul each well)
SD5009	Bio Basic	12UNIT	EUR 313.16

96-well plate Plasmid DNA Prep (mini)
TBS6012	Tribioscience	2x 96-well Plates	EUR 442

96 Well PCR Plate Clear Wells - PK50
PCR0972	Scientific Laboratory Supplies	PK50	EUR 290.25

Random Access 96 Well Plate Skir - PK50
PCR1412	Scientific Laboratory Supplies	PK50	EUR 338.85

96-Well Plate Viral DNA Miniprep Kit
VT92032	Bio Basic	2XPlates, 192prep	EUR 495.86

96-Well Plate Plasmid DNA Miniprep Kit
BS415	Bio Basic	5XPlates, 480prep	EUR 300.37

Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTAG75-5P	Alpha Diagnostics	1 PK	EUR 343.2

96-Well Plate DNA Cleanup Miniprep Kit
BS369	Bio Basic	5XPlates, 480prep	EUR 300.37

Falcon Microtest Black 96 Well Plate - PK32
353376	Scientific Laboratory Supplies	PK32	EUR 244.35

DNA Coating Solution, with 96-well Plate
AKR-5182	Cell Biolabs	10mL	EUR 132
Description: N/A

VersiPlate, 96-well PCR Plate - 25 plates
AB-1800	Westburg	each	EUR 149.88

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)
CT366	U-CyTech	5	EUR 268.8

Benchmark Beadblaster 96 Adapter for 96 Well Plate - PK2
HOM3208	Scientific Laboratory Supplies	PK2	EUR 1923.75

96-Well Plate PCR Products Purification Kit
BS365	Bio Basic	5XPlates, 480prep	EUR 300.37

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED
P-DW-12-HC-S	CORNING	5/pk	EUR 248.4
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

96 Well ELISA Plate, Undetachable, High Binding, Clear Frame & CLear Well, 5/pk,
514201	NEST Biotechnology	50 pcs/cs	EUR 30.36
Description: 96 Well ELISA Plate, Undetachable, High Binding, Clear Frame & CLear Well, 5/pk, 50/cs

96 Well ABI Fast Plate Pcr - PK50
PCR1200	Scientific Laboratory Supplies	PK50	EUR 290.25

96 WELL CLEAR PCR PLATE FOR ABI
PCR-96-AB-C	CORNING	10/pk	EUR 200.4
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 Deep Well Plate Round Wells U - PK100
PCR0228	Scientific Laboratory Supplies	PK100	EUR 244.35

96 Deep Well Plate Round Wells V - PK100
PCR0230	Scientific Laboratory Supplies	PK100	EUR 244.35

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)
EXOEL-96P-1	SBI	10 Plates	EUR 255.6

Benchmark Beadblaster 96 Eva Cap Mat for 96 Well Deep Well Plate - EACH
HOM3232	Scientific Laboratory Supplies	EACH	EUR 136.35

Plate 96 Well Immulon 1 FB - PK50
N3355	Scientific Laboratory Supplies	PK50	EUR 233.55

Bovine fetuin coated ELISA plates (8x12 wells/plate; 5 plates/pk)
800-181-5P	Alpha Diagnostics	1 pk	EUR 343.2

CORNING® BIOCOAT™ GELATIN 96 WELL CLEAR FLAT BOTTOM ASSAY PLATE, WITH LID, NONSTERILE, 5/PACK, 50/CASE
356689	CORNING	5/pk	EUR 800.4
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

Deep-Multiple Well Plate 96 wells, U ty
DM1-50NO	EWC Diagnostics	1 unit	EUR 483.15
Description: Deep-Multiple Well Plate 96 wells, U ty

Deep-Multiple Well Plate 96 wells, U ty
DM4-24NO	EWC Diagnostics	1 unit	EUR 295.56
Description: Deep-Multiple Well Plate 96 wells, U ty

Deep-Multiple Well Plate 96 wells, U ty
DM4-48NO	EWC Diagnostics	1 unit	EUR 572.66
Description: Deep-Multiple Well Plate 96 wells, U ty

Nunc Lid for 96 Well Plate SH - PK100
263339	Scientific Laboratory Supplies	PK100	EUR 126.9

96 Well PCR Plate Divisible In 8
PCR1062	Scientific Laboratory Supplies	PK50	EUR 154.44

Bovine Collagen Coated 6 Well Plates
IBOCLGMH5EA	Innovative research	each	EUR 129

Description: Bovine Collagen Coated 6 Well Plates

Bovine Collagen Coated 48 Well Plates
IBOCLGMM5EA	Innovative research	each	EUR 135

Description: Bovine Collagen Coated 48 Well Plates

Adapter for 96 well plate, pack of 2
IPD9600-96	Benchmark Scientific	each	EUR 1337.97

96 Well Roche Style Pcr Plate - PK50
PCR1214	Scientific Laboratory Supplies	PK50	EUR 330.75

PCR Plate Non Skirted 96 Well - PK50
MOL2364	Scientific Laboratory Supplies	PK50	EUR 348.3

Block for 96 well plate, WSC-2620/30
4002659	Atto	1unit	EUR 458.4

Presto™ RNA 96 Well Binding Plate
96RBP005	Geneaid Biotech	each	EUR 132

Presto™ RNA 96 Well Binding Plate
96RBP100	Geneaid Biotech	each	EUR 2200

i-poly Cell Culture Plate (96 well)
IPY-31096	iNtRON Biotechnology Inc	50 ea/cs	EUR 33
Description: Type: Plate

96 Well Roche Style Qpcr Plate - PK50
PCR1210	Scientific Laboratory Supplies	PK50	EUR 318.6

PCR Plate Semi Skirted 96 Well - PK50
MOL2366	Scientific Laboratory Supplies	PK50	EUR 338.85

CORNING® BIOCOAT™ T-CELL ACTIVATION CONTROL 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE
354730	CORNING	5/pk	EUR 102
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

Presto™ gDNA 96 Well Binding Plate
96GBP005	Geneaid Biotech	each	EUR 103

Presto™ gDNA 96 Well Binding Plate
96GBP100	Geneaid Biotech	each	EUR 1854

96 Well Lung Tumor Genomic DNA Plate
D8235152-1	Biochain	1 plate	EUR 276.85

96 Well Skirted Plate+ Q-Stick - PK50
PCR1238	Scientific Laboratory Supplies	PK50	EUR 311.85

96-Well ROCK Activity Assay Kit
MBS168354-5x96Assays	MyBiosource	5x96Assays	EUR 3810

96-Well ROCK Activity Assay Kit
MBS168354-96Assays	MyBiosource	96Assays	EUR 975

96-Well ROCK Activity Assay Kit
STA-416	Cell Biolabs	96 assays	EUR 624
Description: Excepted Quantities

96-Well ROCK Activity Assay Kit
STA-416-5	Cell Biolabs	5 x 96 assays	EUR 2700
Description: Excepted Quantities

Presto™ Plasmid 96 Well Filter Plate
96PFP005	Geneaid Biotech	each	EUR 78.5

Presto™ Plasmid 96 Well Filter Plate
96PFP100	Geneaid Biotech	each	EUR 1415

96-Well G-25 Gel Filtration Plate
96CG02	Geneaid Biotech	each	EUR 250

96-Well G-25 Gel Filtration Plate
96CG04	Geneaid Biotech	each	EUR 475

96-Well G-25 Gel Filtration Plate
96CG10	Geneaid Biotech	each	EUR 1065

96-Well G-50 Gel Filtration Plate
CGP02	Geneaid Biotech	each	EUR 250

96-Well G-50 Gel Filtration Plate
CGP04	Geneaid Biotech	each	EUR 589.6

96-Well G-50 Gel Filtration Plate
CGP10	Geneaid Biotech	each	EUR 1382

96 Well Colon Tumor Genomic DNA Plate
D8235090-1	Biochain	1 plate	EUR 276.85

S100 96-well PCR Plate Adapter - EACH
E5391070026	Scientific Laboratory Supplies	EACH	EUR 426.6

S200 96-well PCR Plate Adapter - EACH
E5392070038	Scientific Laboratory Supplies	EACH	EUR 426.6

96 Well PCR Plate Divisible In12 - PK50
PCR1064	Scientific Laboratory Supplies	PK50	EUR 195.75

96 Well Semi-Skirted PCR Plate - PK50
PCR1106	Scientific Laboratory Supplies	PK50	EUR 414.45

Presto™ Plasmid 96 Well Binding Plate
96PBP005	Geneaid Biotech	each	EUR 78.5

Presto™ Plasmid 96 Well Binding Plate
96PBP100	Geneaid Biotech	each	EUR 1415

96 Well Breast Tumor Genomic DNA Plate
D8235086-1	Biochain	1 plate	EUR 276.85

96 Well Impact MicroBatch Plate, 80/CSE
M-673101	MiTeGen	80 PLATES	EUR 808
Description: 96 Well Impact MicroBatch Plate, 80/CSE

96 Well Solid Bottom Plate Black - PK100
PCR0354	Scientific Laboratory Supplies	PK100	EUR 475.2

96 Well Solid Bottom Plate White - PK100
PCR0360	Scientific Laboratory Supplies	PK100	EUR 437.4

OxiSelect 96-Well Comet Assay Kit
MBS168155-5x96Assays	MyBiosource	5x96Assays	EUR 1815

OxiSelect 96-Well Comet Assay Kit
MBS168155-96Assays	MyBiosource	96Assays	EUR 510

Olympus 96-Well PCR Plate, FAST-type
24-310	Genesee Scientific	25 Plates/Unit	EUR 57.82
Description: Low Profile, Natural

Olympus 96-Well PCR Plate, FAST-type
24-310W	Genesee Scientific	10 Plates/Unit	EUR 29.37
Description: Low Profile, White

96 Well Stomach Tumor Genomic DNA Plate
D8235248-1	Biochain	1 plate	EUR 276.85

Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)
M-R-1008649	MiTeGen	98 X 1.7 ml	EUR 316
Description: Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)

96 WELL PLATE; SQUARE; U BASE; 1.6ML; PP; 96/PK - PK96
60180-P155	Scientific Laboratory Supplies	PK96	EUR 631.8

96-Well Skirted PCR Plate, Red 25 plates
MU23090	Westburg	each	EUR 124.81

Mouse anti-ACTH Clear Coated 96 Well Plate
C271-1EA	Arbor Assays	1EA	EUR 150

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031	Bio Basic	2Plates, 192prep	EUR 210.85

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032	Bio Basic	5Plates, 480prep	EUR 389.9

96-Well Skirted PCR Plate, Blue 25 plates
MU23100	Westburg	each	EUR 124.81

OxiSelect 96-Well Comet Assay Slide
MBS168033-1Slide	MyBiosource	1Slide	EUR 245

OxiSelect 96-Well Comet Assay Slide
MBS168033-5Slides	MyBiosource	5Slides	EUR 595

OxiSelect 96-Well Comet Assay Slide
MBS168033-5x5Slides	MyBiosource	5x5Slides	EUR 2500

Radius 96-Well Cell Migration Assay
MBS168787-5x96Assays	MyBiosource	5x96Assays	EUR 2525

Radius 96-Well Cell Migration Assay
MBS168787-96Assays	MyBiosource	96Assays	EUR 680

Olympus 96-Well PCR Plate, Non-Skirted
24-300	Genesee Scientific	25 Plates/Unit	EUR 57.13
Description: Ultra Thin Wall, Natural

96-Well Skirted PCR Plate, Green 25 plates
MU23110	Westburg	each	EUR 124.81

96-Well Skirted PCR Plate, Black 25 plates
MU37360	Westburg	each	EUR 136.25

96-Well Skirted PCR Plate, White 25 plates
MU37370	Westburg	each	EUR 136.25

Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
M-R-1008647	MiTeGen	96 X 1.7 ml	EUR 316
Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
M-R-1008648	MiTeGen	97 X 1.7 ml	EUR 316
Description: Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

96-Well Skirted PCR Plate, Yellow 25 plates
MU23120	Westburg	each	EUR 124.81

Olympus 96-Well PCR Plate, Semi-Skirted
24-301	Genesee Scientific	25 Plates/Unit	EUR 57.82
Description: Raised Rim, Natural

Olympus 96-Well PCR Plate, Full-Skirted
24-302	Genesee Scientific	25 Plates/Unit	EUR 60.31
Description: Ultra Thin Wall, Natural

Olympus 96-Well PCR Plate, Full-Skirted
24-302W	Genesee Scientific	10 Plates/Unit	EUR 27.75
Description: Ultra Thin Wall, White

Olympus 96-Well PCR Plate, Semi-Skirted
27-408	Genesee Scientific	10 Plates/Unit	EUR 23.76
Description: Straight-Sided, Natural

Olympus 96-Well PCR Plate, Semi-Skirted
27-108	Genesee Scientific	10 Plates/Unit	EUR 23.71
Description: Flat Rim, Natural

96-well Nucleocuvette Plate (via MTA only)
LOVHNP-1001	Westburg	each	EUR 275.45

Total RNA Purification Kit - 96 Well Plate Format
24300	Norgen Biotek Corp	2 Plates	EUR 419.4

Total RNA Purification Kit - 96 Well Plate Format
24370	Norgen Biotek Corp	6 Plates	EUR 1193.4

96-Well Plate Animal Genomic DNA Miniprep Kit
BS437	Bio Basic	5XPlates, 480prep	EUR 435.58

96-Well Plate Animal Genomic DNA Miniprep Kit
BS4372	Bio Basic	2XPlates, 192prep	EUR 234.6

96 Well Plate RNA Cleanup and Concentration Kit
BRC1251	Bio Basic	2xPlates, 192prep	EUR 300.37

96-Well Skirted PCR Plate, Natural 25 plates
MU23080	Westburg	each	EUR 129.71

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE-96001	Favorgen	1 prep	EUR 175.2

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE_96001	Favorgen	1 kit	EUR 36

VersiPlate, 96-well PCR Plate White - 25 plates
AB-1800/W	Westburg	each	EUR 169.5

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1292	Bio Basic	2XPlates, 192prep	EUR 210.85

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1295	Bio Basic	5XPlates, 480prep	EUR 389.9

96 WELL PLATE; SQUARE; 2ML; PP; 1/PK - EACH
60180-P403	Scientific Laboratory Supplies	EACH	EUR 21.6

EVA Cap Mat for 96 well deep well plate, DNase/RNase Free
IPD1196-CM	Benchmark Scientific	each	EUR 92.08

Olympus 96-well Cuttable PCR Plate, Unskirted
24-303C	Genesee Scientific	10 Plates/Unit	EUR 30.12
Description: Polypropylene, Natural

2 x Black Half Area 96 Well Plate, 2EA
X037-2EA	Arbor Assays	2EA	EUR 37

MULTIWELL PLATE POLYSTYRENE 96-WELL FLA - PK100
M2936-100EA	Scientific Laboratory Supplies	PK100	EUR 148.35

PCR Plate 96 Well No Skirt Nat - PK100
LW2200	Scientific Laboratory Supplies	PK100	EUR 600.75

CytoSelect 96-well Cell Transformation Assay
MBS169037-5x96Assays	MyBiosource	5x96Assays	EUR 3200

CytoSelect 96-well Cell Transformation Assay
MBS169037-96Assays	MyBiosource	96Assays	EUR 845

96 Well PCR Plate Divisible In 8 - PK50
PCR1060	Scientific Laboratory Supplies	PK50	EUR 195.75

NEST MagPure 0.5 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible
500021	NEST Biotechnology	50 pcs/cs	EUR 31.68
Description: NEST MagPure 0.5 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible with Kingfiser Flex&Presto, Non-Sterile, 5/pk, 50/cs

NEST MagPure 0.5 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible
500621	NEST Biotechnology	50 pcs/cs	EUR 31.57
Description: NEST MagPure 0.5 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible with Kingfiser Flex&Presto, Sterile, 5/pk, 50/cs

CytoSelect 96-well Phagocytosis Assay (Zymosan)
MBS168370-5x96Assays	MyBiosource	5x96Assays	EUR 3170

CytoSelect 96-well Phagocytosis Assay (Zymosan)
MBS168370-96Assays	MyBiosource	96Assays	EUR 820

UNIFILTER PLATE 96 WELL 800 UL/PER WEL - EACH
WHA77002801	Scientific Laboratory Supplies	EACH	EUR 1098.9

Thermo Fast 96 Well PCR Plate - PK25 - PK25
AB0800	Scientific Laboratory Supplies	PK25	EUR 286.2

96-Well Plate Template Post-It Notes, 4"x6"
6290	Immunochemistry	100 sheets	EUR 7.8

96-Well Plate Template Post-It Notes, 4"x6"
MBS258083-100Sheets	MyBiosource	100Sheets	EUR 115

96-Well Plate Template Post-It Notes, 4"x6"
MBS258083-5x100Sheets	MyBiosource	5x100Sheets	EUR 340

Attachment of the antigens to the ELISA floor utilizing a layer of anti-histidine antibodies gave equal decision for each S-RBD_N318-V510 and the full-length spike protein. Outcomes exhibit that ELISA-functional SARS-CoV-2 antigens could be produced in bacterial cultures, and that S-RBD_N318-V510 could symbolize a cheap different to using structurally extra advanced antigens in serological COVID-19 testing.