Production of high-quality SARS-CoV-2 antigens: impact of bioprocess and storage on glycosylation, biophysical attributes, and ELISA serologic tests performance

June 23, 2021 Taylor 0 Comments

Serological assays are helpful instruments to check SARS-CoV-2 unfold and, importantly, to determine people that have been already contaminated and could be probably resistant to a virus re-infection. SARS-CoV-2 Spike protein and its Receptor Binding Area (RBD) are the antigens with larger potential to develop SARS-CoV-2 serological assays. Furthermore, structural research of those antigens are key to know the molecular foundation for Spike interplay with angiotensin changing enzyme 2 receptor, hopefully enabling the event of COVID-19 therapeutics.

Thus, it’s pressing that vital quantities of this protein grew to become accessible on the highest high quality. On this work, we produced Spike and RBD in two human derived cell hosts: HEK293-E6 and Expi293F™. We evaluated the affect of various and scalable bioprocessing approaches on Spike and RBD manufacturing yields and, extra importantly, on these antigens’ high quality attributes. Utilizing destructive and optimistic sera collected from human donors, we present a wonderful efficiency of the produced antigens, assessed in serologic ELISA exams, as denoted by the excessive specificity and sensitivity of the check.

We present sturdy Spike productions with last yields of approx. 2 mg per liter of tradition that have been maintained independently of the manufacturing scale or cell tradition technique. To the most effective of our data, the ultimate yield of 90 mg per liter of tradition obtained for RBD manufacturing, was the best reported so far. An in-depth characterization of SARS-CoV-2 Spike and RBD proteins was carried out, specifically the antigen’s oligomeric state, glycosylation profiles and thermal stability throughout storage.

The correlation of those high quality attributes with ELISA efficiency present equal reactivity to SARS-CoV-2 optimistic serum, for all Spike and RBD produced, and for all storage circumstances examined. Total, we offer simple protocols to provide high-quality SARS-CoV-2 Spike and RBD antigens, that may be simply tailored to each tutorial and industrial settings; and combine, for the primary time, research on the affect of bioprocess with an in-depth characterization of those proteins, correlating antigen’s glycosylation and biophysical attributes to efficiency of COVID-19 serologic exams.

Analysis of IgM LAT and IgM ELISA as in comparison with microscopic agglutination check (MAT) for early analysis of Leptospira sp

Leptospirosis is a worldwide zoonotic illness attributable to spirochetes of the genus Leptospira. The scientific manifestation of leptospirosis is non-specific and continuously misdiagnosed as different sicknesses. The purpose of this research was to match the diagnostic accuracies of two industrial exams for early analysis of Leptospira species: the IgM latex agglutination check (IgM LAT) and the IgM enzyme-linked immunosorbent assay (IgM ELISA).

A complete of 140 serum samples have been obtained from sufferers suspected of leptospirosis on the Universiti Kebangsaan Malaysia Medical Centre (UKMMC). These serum samples have been examined for the presence of Leptospira sp. utilizing IgM LAT, IgM ELISA and MAT. From Desk 1, IgM LAT confirmed 21% (n = 29) optimistic, 18% (n = 25) inconclusive and 61% (n = 86) destructive, whereas IgM ELISA confirmed 6% (n = 8) optimistic, 6% (n = 8) inconclusive, 88% (n = 124) destructive and MAT confirmed 11% (n = 16) optimistic, 47% (n = 65) inconclusive, 42% (n = 59) destructive.

The sensitivity, specificity, optimistic predictive worth (PPV) and destructive predictive worth (NPV) of IgM LAT have been 68.8%, 57.6%, 30.6% and 87.2% respectively, whereas for IgM ELISA they have been 37.5%, 89.8%, 50% and 84.1%, respectively as in comparison with MAT (Desk 2). The outcomes confirmed that IgM LAT had larger sensitivity however decrease specificity in comparison with IgM ELISA.

In conclusion, IgM LAT could be helpful as an early screening check for early analysis of Leptospira sp., whereas IgM ELISA is an acceptable methodology for decreasing false destructive detection of Leptospira sp. As each exams present reasonable percentages (~65%) in accuracy, a further check is required for higher detection of Leptospira sp.

Seroprevalence of Toxoplasma gondii in ruminants through the use of latex agglutination check (LAT) and enzyme-linked immunosorbent assay (ELISA) in Assiut governorate

Toxoplasmosis is a crucial zoonotic parasitic illness world-wide. Nonetheless, knowledge on Toxoplasma gondii an infection in ruminants are scarce in Assiut governorate, Egypt. Thus we carried out a survey on the seroprevalence of T. gondii an infection through the use of latex agglutination check (LAT) and enzyme-linked immunosorbent assay (ELISA). A complete of 274 sera have been examined (56 imported camels, 56 cattle, 55 buffaloes, 50 sheep and 57 goats) within the interval from November 2014 – February 2016.

The general seroprevalence in ruminants was (35.8%) by LAT and (83.6%) by ELISA. The very best charge of T. gondii an infection by LAT was present in goats (47.4%) adopted by sheep (44%), camels (35.7%), cattle (32.1%) and buffaloes (20%). The very best charge of T. gondii an infection by ELISA was recorded in camels (96.4%) adopted by goats (87.7%), sheep (86%), buffaloes (74.5%) and cattle (73.2%). Antibody titres to T. gondii in optimistic sera by LAT ranged from 1:2 to 1:64 in imported camels, 1:2 to 1:32 in cattle, 1:2 to 1:Eight in buffaloes, from 1:Four to 1:64 in sheep and from 1:2 to 1:16 in goats. The variations of seroprevalence between completely different species have been vital by LAT and extremely vital by ELISA. Our outcomes counsel that the T. gondii parasite is extensively unfold on account of excessive publicity to infective cat faeces.

Serological Check to Decide Publicity to SARS-CoV-2: ELISA Based mostly on the Receptor-Binding Area of the Spike Protein (S-RBD _N318-V510) Expressed in Escherichia coli

Huge worldwide serological testing for SARS-CoV-2 is required to find out the extent of virus publicity in a selected area, the ratio of symptomatic to asymptomatic contaminated individuals, and the period and extent of immunity after an infection. To realize this, the event and manufacturing of dependable and cost-effective SARS-CoV-2 antigens is vital. We report the bacterial manufacturing of the peptide S-RBD_N318-V510, which incorporates the receptor-binding area of the SARS-CoV-2 spike protein (area of 193 amino acid residues from asparagine-318 to valine-510) of the SARS-CoV-2 spike protein.

We purified this peptide utilizing an easy method involving bacterial lysis, his-tag-mediated affinity chromatography, and imidazole-assisted refolding. The antigen performances of S-RBD_N318-V510 and a industrial full-length spike protein have been in contrast in ELISAs. In direct ELISAs, the place the antigen was instantly sure to the ELISA floor, each antigens discriminated sera from non-exposed and uncovered people. Nonetheless, the discriminating decision was higher in ELISAs that used the full-spike antigen than the S-RBD_N318-V510.

Black 96 Well Plate, 1EA
X025-1EA	Arbor Assays	1EA	EUR 87.6

96 Well Semi Skirted Plate
PCR1216	Scientific Laboratory Supplies	PK50	EUR 150.48

96 Well Skirted PCR Plate
PCR1236	Scientific Laboratory Supplies	PK50	EUR 299.82

96-Well Protein Folding Plate
PFS096	ProFoldin	kit	EUR 347.84
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins.

Streptavidin coated 96-well Plate
6523-5	Biovision	each	EUR 392.4

96 well filter plate (960ul each well)
SD5006	Bio Basic	12UNIT	EUR 115.85

Deep Well Plate 96 Well 2.0 ML
AXYPDW20C	Scientific Laboratory Supplies	PK50	EUR 181.26

96 Deep Well Plate Square Well V
PCR0246	Scientific Laboratory Supplies	PK50	EUR 258.78

Brand 96 Well Deep Well Plate-Stackable
BR701354-24EA	Scientific Laboratory Supplies	PK24	EUR 144.78

UNIFILTER PLATE 96 WELL 800 UL/WELL
WHA77002803	Scientific Laboratory Supplies	PK25	EUR 823.08

96 WELL CLEAR PCR PLATE FOR ABI
PCR-96-AB-C	CORNING	10/pk	EUR 200.4
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY
P-DW-12-HC	CORNING	5/pk	EUR 182.4
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

DEEP WELL PLATE 96 WELL 2.0 ML STERIL
AXYPDW20CS	Scientific Laboratory Supplies	PK50	EUR 216.6

Clear Coated 96 Well Plate, 1EA
C107-1EA	Arbor Assays	1EA	EUR 226.8

96 well filter plate (no filter)
SD5006-EMPTY	Bio Basic	12UNIT	EUR 104.89

Falcon Microtest Black 96 Well Plate
353376	Scientific Laboratory Supplies	PK32	EUR 199.5

Nunc Lid for 96 well plate
264122	Scientific Laboratory Supplies	PK50	EUR 116.28

MULTIWELL PLATE POLYSTYRENE 96-WELL FLA
M2936-100EA	Scientific Laboratory Supplies	PK100	EUR 115.14

PCR Plate Non Skirted 96 Well
MOL2364	Scientific Laboratory Supplies	PK50	EUR 259.69

PCR Plate Semi Skirted 96 Well
MOL2366	Scientific Laboratory Supplies	PK50	EUR 251.83

Plate 96 Well Immulon 1 FB
N3355	Scientific Laboratory Supplies	PK50	EUR 177.84

S100 96-well PCR Plate Adapter
E5391070026	Scientific Laboratory Supplies	EACH	EUR 335.16

S200 96-well PCR Plate Adapter
E5392070038	Scientific Laboratory Supplies	EACH	EUR 335.16

96 Deep Well Plate Square Wells
PCR0240	Scientific Laboratory Supplies	PK100	EUR 270.18

96 Deep Well Plate Round Wells
PCR0242	Scientific Laboratory Supplies	PK50	EUR 232.56

96 Deep Well Plate Square WellU
PCR0256	Scientific Laboratory Supplies	PK50	EUR 180.12

96 Well Solid Bottom Plate Black
PCR0354	Scientific Laboratory Supplies	PK100	EUR 343.14

96 Well Solid Bottom Plate White
PCR0360	Scientific Laboratory Supplies	PK100	EUR 313.5

96 Well PCR Plate Clear Wells
PCR0972	Scientific Laboratory Supplies	PK50	EUR 201.78

96 Well PCR Plate Divisible In12
PCR1064	Scientific Laboratory Supplies	PK50	EUR 128.82

96 Well Semi-Skirted PCR Plate
PCR1106	Scientific Laboratory Supplies	PK50	EUR 285

96 Well ABI Fast Plate Pcr
PCR1200	Scientific Laboratory Supplies	PK50	EUR 201.78

96 Well Roche Style Qpcr Plate
PCR1210	Scientific Laboratory Supplies	PK50	EUR 223.44

96 Well Roche Style Pcr Plate
PCR1214	Scientific Laboratory Supplies	PK50	EUR 233.7

96 Well Roche Style PCR Plate
PCR1218	Scientific Laboratory Supplies	PK50	EUR 213.18

96 Well Skirted Plate+ Q-Stick
PCR1238	Scientific Laboratory Supplies	PK50	EUR 218.88

Random Access 96 Well Plate Skir
PCR1412	Scientific Laboratory Supplies	PK50	EUR 233.7

INTELLI-PLATE 96-2 Shallow well
MAR-102-0001-20	MiTeGen	120 PLATES	EUR 990

Description: INTELLI-PLATE 96-2 Shallow well; 2-well sitting drop, 120 plates

24 Well Solid Bottom Assay Plate
PCR0352	Scientific Laboratory Supplies	PK100	EUR 373.92

Benchmark Beadblaster 96 Adapter for 96 Well Plate
HOM3208	Scientific Laboratory Supplies	PK2	EUR 1423.86

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.
CP-AXYGEM-96-50	CORNING	10/pk	EUR 548.4
Description: Crystallography Plates; Crystallography Plates - Axygen

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE
PCR-96-HS-C	CORNING	10/pk	EUR 190.8
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Bovine Immunoglobulin M (IgM) ELISA Kit
DLR-IgM-b-48T	DL Develop	48T	EUR 547.2

Description: A sandwich quantitative ELISA assay kit for detection of Bovine Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Immunoglobulin M (IgM) ELISA Kit
DLR-IgM-b-96T	DL Develop	96T	EUR 708

Description: A sandwich quantitative ELISA assay kit for detection of Bovine Immunoglobulin M (IgM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Immunoglobulin M (IgM) ELISA Kit
RDR-IgM-b-48Tests	Reddot Biotech	48 Tests	EUR 567.6

Bovine Immunoglobulin M (IgM) ELISA Kit
RDR-IgM-b-96Tests	Reddot Biotech	96 Tests	EUR 784.8

Bovine Immunoglobulin M (IgM) ELISA Kit
RD-IgM-b-48Tests	Reddot Biotech	48 Tests	EUR 543.6

Bovine Immunoglobulin M (IgM) ELISA Kit
RD-IgM-b-96Tests	Reddot Biotech	96 Tests	EUR 750

96 well DNA plate with membrane (960ul each well)
SD5007	Bio Basic	12UNIT	EUR 291.24

96 well RNA plate with membrane (960ul each well)
SD5009	Bio Basic	12UNIT	EUR 313.16

Benchmark Beadblaster 96 Eva Cap Mat for 96 Well Deep Well Plate
HOM3232	Scientific Laboratory Supplies	EACH	EUR 98.04

Radius 96-Well Cell Migration Assay
CBA-126	Cell Biolabs	96 assays	EUR 686.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay
CBA-126-5	Cell Biolabs	5 x 96 assays	EUR 2697.6
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay
CBA-130	Cell Biolabs	96 assays	EUR 866.4
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay
CBA-130-5	Cell Biolabs	5 x 96 assays	EUR 3463.2
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224	Cell Biolabs	96 assays	EUR 866.4
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224-5	Cell Biolabs	5 x 96 assays	EUR 3525.6
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

OxiSelect 96-Well Comet Assay Kit
STA-355	Cell Biolabs	96 assays	EUR 512.4
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit
STA-355-5	Cell Biolabs	5 x 96 assays	EUR 1959.6
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Slide
STA-356	Cell Biolabs	1 slide	EUR 219.6
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide
STA-356-5	Cell Biolabs	5 slides	EUR 622.8
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

96-Well ROCK Activity Assay Kit
STA-416	Cell Biolabs	96 assays	EUR 1027.2
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit
STA-416-5	Cell Biolabs	5 x 96 assays	EUR 4228.8
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96 Well Breast Tumor Genomic DNA Plate
D8235086-1	Biochain	1 plate	EUR 379.2

96 Well Colon Tumor Genomic DNA Plate
D8235090-1	Biochain	1 plate	EUR 379.2

96 Well Lung Tumor Genomic DNA Plate
D8235152-1	Biochain	1 plate	EUR 379.2

96 Well Stomach Tumor Genomic DNA Plate
D8235248-1	Biochain	1 plate	EUR 379.2

96-Well Plate PCR Products Purification Kit
BS365	Bio Basic	5XPlates, 480prep	EUR 300.37

96-Well Plate PCR Products Purification Kit
BS3652	Bio Basic	2xPlates, 192prep	EUR 186.19

96-Well Plate DNA Cleanup Miniprep Kit
BS369	Bio Basic	5XPlates, 480prep	EUR 300.37

96-Well Plate DNA Cleanup Miniprep Kit
BS3692	Bio Basic	2xPlates, 192prep	EUR 186.19

96-Well Plate Plasmid DNA Miniprep Kit
BS415	Bio Basic	5XPlates, 480prep	EUR 300.37

96-Well Plate Plasmid DNA Miniprep Kit
BS4152	Bio Basic	2XPlates, 192prep	EUR 186.19

Polystyrene-bottomed 96-well plate + lid (ELISPOT)
CT365	U-CyTech	5	EUR 93.6

96-Well Plate Viral DNA Miniprep Kit
VT92032	Bio Basic	2XPlates, 192prep	EUR 495.86

96-well PCR Plate Non-Skirted natural
AB-0600	Scientific Laboratory Supplies	PK25	EUR 160.74

96-well PCR Plate Non-Skirted (White)
AB-0600W	Scientific Laboratory Supplies	PK25	EUR 191.52

96-well PCR Plate (Segmented Semi-Skirted)
AB-0900	Scientific Laboratory Supplies	PK25	EUR 159.6

SuperPlate 96-well PCR Plate Semi-Skirted
AB-2400	Scientific Laboratory Supplies	PK25	EUR 207.48

Thermo Fast 96 Well PCR Plate - PK25
AB0800	Scientific Laboratory Supplies	PK25	EUR 225.72

Nunc Lid for 96 Well Plate SH
263339	Scientific Laboratory Supplies	PK100	EUR 102.6

PCR Plate 96 Well No Skirt Nat
LW2200	Scientific Laboratory Supplies	PK100	EUR 452.58

MULTIWELL PLATE, POLYSTYRENE 96-WELL U-B
M2186-100EA	Scientific Laboratory Supplies	PK100	EUR 115.14

2 x Clear 96 well Plate, 2EA
X003-2EA	Arbor Assays	2EA	EUR 78

5 x Clear 96 well Plate, 5EA
X003-5EA	Arbor Assays	5EA	EUR 96

2 x Clear 96 well Plate, 2EA
X018-2EA	Arbor Assays	2EA	EUR 78

4 x Clear 96 Well Plate, 4EA
X018-4EA	Arbor Assays	4EA	EUR 104.4

Black Half Area 96 Well Plate, 1EA
X023-1EA	Arbor Assays	1EA	EUR 96

96 Deep Well Plate Round Wells U
PCR0228	Scientific Laboratory Supplies	PK100	EUR 166.44

96 Deep Well Plate Round Wells V
PCR0230	Scientific Laboratory Supplies	PK100	EUR 166.44

96 Well PCR Plate Divisible In 8
PCR1060	Scientific Laboratory Supplies	PK50	EUR 128.82

96 Well PCR Plate Divisible In 8
PCR1062	Scientific Laboratory Supplies	PK50	EUR 140.22

96 Well Impact MicroBatch Plate, 80/CSE
M-673101	MiTeGen	80 PLATES	EUR 808

Description: 96 Well Impact MicroBatch Plate, 80/CSE

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)
ACT-SL-96	ACTGene	each	EUR 776.4

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED
P-DW-12-HC-S	CORNING	5/pk	EUR 248.4
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

96 WELL PLATE; SQUARE; U BASE; 1.6ML; PP; 96/PK
60180-P155	Scientific Laboratory Supplies	PK96	EUR 484.5

Benchmark Beadblaster 96 96 Well Plate Prefilled with 4mm Beads
HOM3228	Scientific Laboratory Supplies	PK10	EUR 2199.06

Bovine IgM ELISA Kit, 96 tests, Quantitative
8080	Alpha Diagnostics	1 Kit	EUR 854.4

CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric)
CBA-081	Cell Biolabs	96 assays	EUR 727.2
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

96-well Checkpoint Kinase Activity Assay Kit
STA-414	Cell Biolabs	96 assays	EUR 908.4
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit
STA-414-5	Cell Biolabs	5 x 96 assays	EUR 3672
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

Block for 96 well plate, WSC-2620/30
4002659	Atto	1unit	EUR 458.4
Description: Bl ock for WSC-2620 / 2630

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031	Bio Basic	2Plates, 192prep	EUR 210.85

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032	Bio Basic	5Plates, 480prep	EUR 389.9

96 Well Plate RNA Cleanup and Concentration Kit
BRC1251	Bio Basic	2xPlates, 192prep	EUR 300.37

96-Well Plate Animal Genomic DNA Miniprep Kit
BS437	Bio Basic	5XPlates, 480prep	EUR 435.58

96-Well Plate Animal Genomic DNA Miniprep Kit
BS4372	Bio Basic	2XPlates, 192prep	EUR 234.6

96 Well Plate Yeast Genomic DNA Miniprep Kit
BS8357	Bio Basic	2XPlates, 192prep	EUR 234.6

96-Well Plate Plant Genomic DNA Miniprep Kit
BS8361	Bio Basic	2XPlates, 192prep	EUR 234.6

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)
CT366	U-CyTech	5	EUR 268.8

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE-96001	Favorgen	1 prep	EUR 175.2

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1292	Bio Basic	2XPlates, 192prep	EUR 210.85

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1295	Bio Basic	5XPlates, 480prep	EUR 389.9

96 WELL PLATE; SQUARE; 2ML; PP; 1/PK
60180-P403	Scientific Laboratory Supplies	EACH	EUR 17.1

96-well PCR Plate (Non-Skirted+black lettering)
AB-0600-L	Scientific Laboratory Supplies	PK25	EUR 168.72

96-well Low Profile PCR Plate Non-Skirted
AB-0700	Scientific Laboratory Supplies	PK25	EUR 171

Abgene Thermo 96 Well PCR Plate Skirted Blue
AB0800B	Scientific Laboratory Supplies	PK25	EUR 245.1

Culture Plate 96 Well Non Treated Ind. Wrapped
ARG1476	Scientific Laboratory Supplies	PK100	EUR 302.1

Tissue Culture Plate 96 Well Treated Ind. Wrapped
ARG1492	Scientific Laboratory Supplies	PK100	EUR 362.52

Nunc 96 Well Glass OPB Plate non-treated
265300	Scientific Laboratory Supplies	PK30	EUR 3098.52

Nunc 96 Well OBP Plate Black non-treated
265301	Scientific Laboratory Supplies	PK30	EUR 416.1

Nunc 96 Well OBP Plate White non-treated
265302	Scientific Laboratory Supplies	PK30	EUR 568.86

Microbiol clear skirted PCR plate 96 well 10pcs
E0030129300	Scientific Laboratory Supplies	EACH	EUR 82.08

Microbiol blue skirted PCR plate 96 well 10pcs
E0030129318	Scientific Laboratory Supplies	EACH	EUR 82.08

PCR plate 96 well unskirted 250ul clear 20pcs
E0030133366	Scientific Laboratory Supplies	EACH	EUR 86.64

PCR plate 96 well unskirted 250ul blue 20pcs
E0030133390	Scientific Laboratory Supplies	EACH	EUR 86.64

Adapter for 96-well PCR plate 2 pcs.
E5825711009	Scientific Laboratory Supplies	PK2	EUR 150.48

BRAND(TM) 96-WELL PCR PLATE SKIRTED WH
BR781378-50EA	Scientific Laboratory Supplies	PK50	EUR 382.93

UNIFILTER PLATE 96 WELL 800 UL/PER WEL
WHA77002801	Scientific Laboratory Supplies	EACH	EUR 823.08

Mouse anti-ACTH Clear Coated 96 Well Plate
C271-1EA	Arbor Assays	1EA	EUR 150

96 Deep Well Masterblock Storage Plate, 50/CSE
M-780261	MiTeGen	50 PLATES	EUR 240

Description: 96 Deep Well Masterblock Storage Plate, 50/CSE

INTELLI-PLATE 96-2 shallow well, low profile
MAR-102-0001-21	MiTeGen	120 PLATES	EUR 990

Description: INTELLI-PLATE 96-2 shallow well, low profile; 2-well sitting drop, 120 plates

Modular In Situ-1 96 Well Crystallization Plate
ML-MIS-FP3D	MiTeGen	1 PLATE	EUR 392

Description: Modular In Situ-1 96 Well Crystallization Plate

Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
M-R-1008647	MiTeGen	96 X 1.7 ml	EUR 316

Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
M-R-1008648	MiTeGen	97 X 1.7 ml	EUR 316

Description: Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)
M-R-1008649	MiTeGen	98 X 1.7 ml	EUR 316

Description: Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)
EXOEL-96P-1	SBI	10 Plates	EUR 255.6

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)
CBA-320	Cell Biolabs	96 assays	EUR 644.4
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit
25-224	Genesee Scientific	100 plates	EUR 358.8
Description: Suitable for cell culture but only for suspension cells Optically clear with low autofluorescence for distortion free microscopy Low evaporation lid reduces evaporation and prevents contamination Exterior stacking ring on lid for added stability when stacking Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML
PCR-96-AB-C-PBC	CORNING	10/pk	EUR 234
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric
CBA-112-COL	Cell Biolabs	96 assays	EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric
CBA-112-LN	Cell Biolabs	96 assays	EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)
CBA-220	Cell Biolabs	96 assays	EUR 762
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96-well Cellular Senescence Assay (SA ?-Gal Activity)
CBA-231	Cell Biolabs	120 assays	EUR 762
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

96-well Cellular Senescence Assay (SA ?-Gal Activity)
CBA-231-5	Cell Biolabs	5 x 120 assays	EUR 3177.6
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTA55-5P	Alpha Diagnostics	1 PK	EUR 315.6

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTG65-5P	Alpha Diagnostics	1 PK	EUR 315.6

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well
1018-96	Intact Genomics	1/EA	EUR 621.6

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480130	CORNING	1/pk	EUR 267.6
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES
480131	CORNING	1/pk	EUR 261.6
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480132	CORNING	1/pk	EUR 261.6
Description: Lab Equipment; Constant Temperature Equipment

Block for 96 deep-well plate, WSC-2620/30
4002661	Atto	1unit	EUR 458.4
Description: Bl ock for WSC-2620 / 2630

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA
C075-1EA	Arbor Assays	1EA	EUR 213.6

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA
C140-1EA	Arbor Assays	1EA	EUR 213.6

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA
C223-1EA	Arbor Assays	1EA	EUR 213.6

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS585	Bio Basic	5XPlates, 480prep	EUR 585.38

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS5852	Bio Basic	2XPlates, 192prep	EUR 300.37

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92028	Bio Basic	2XPlates, 192prep	EUR 254.7

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92029	Bio Basic	5XPlates, 480prep	EUR 437.4

96 Well PCR Plates, Fits ABI Fast Plate Machines
GP615	GenDepot	25 plates	EUR 254.4

Falcon PP 96 Well Round Bottom Library Storage Plate
351190	Scientific Laboratory Supplies	PK100	EUR 345.42

Falcon 96-well 340ul Storage Polypropylene Plate V-bottom
353263	Scientific Laboratory Supplies	PK100	EUR 336.3

Falcon 96 Well White Solid Tissue Culture Treated Plate
353296	Scientific Laboratory Supplies	PK50	EUR 347.7

BD Primaria 96 Well Flat Bottom Tissue Culture Plate
353872	Scientific Laboratory Supplies	PK50	EUR 297.54

BD BioCoat Laminin Coated 96 Well Tissue Culture Plate
354410	Scientific Laboratory Supplies	PK5	EUR 280.44

BD BioCoat Gelatin 96 Well Clear Tissue Culture Plate
354689	Scientific Laboratory Supplies	PK5	EUR 106.02

CoolSink XT 96U 96 well u-bottom plate module
432071	Scientific Laboratory Supplies	EACH	EUR 386.46

96-well PCR Plate (Skirted L Prfl+black lettering)
AB-0800-L	Scientific Laboratory Supplies	PK25	EUR 237.12

96-well PCR Plate Semi-Skirted with Raised Deck
AB-1100	Scientific Laboratory Supplies	PK25	EUR 163.02

Microbiol semi skirted blue PCR plate 96 well 10pcs
E0030129334	Scientific Laboratory Supplies	EACH	EUR 82.08

PCR plate 96 well unskirted low profile clear 20pcs
E0030133307	Scientific Laboratory Supplies	PK20	EUR 86.64

PCR plate 96 well unskirted low profile blue 20pcs
E0030133331	Scientific Laboratory Supplies	EACH	EUR 86.64

Divisible PCR plate 96 well unskirted 250ul blue 20pcs
E0030133404	Scientific Laboratory Supplies	EACH	EUR 155.04

Goat anti-Mouse, Coated White 96 Well Plate, 1EA
X010-1EA	Arbor Assays	1EA	EUR 130.8

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA
X014-1EA	Arbor Assays	1EA	EUR 130.8

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA
X016-1EA	Arbor Assays	1EA	EUR 130.8

Attachment of the antigens to the ELISA floor utilizing a layer of anti-histidine antibodies gave equal decision for each S-RBD_N318-V510 and the full-length spike protein. Outcomes exhibit that ELISA-functional SARS-CoV-2 antigens could be produced in bacterial cultures, and that S-RBD_N318-V510 could symbolize a cheap different to using structurally extra advanced antigens in serological COVID-19 testing.