Production of high-quality SARS-CoV-2 antigens: impact of bioprocess and storage on glycosylation, biophysical attributes, and ELISA serologic tests performance
Serological assays are helpful instruments to check SARS-CoV-2 unfold and, importantly, to determine people that have been already contaminated and could be probably resistant to a virus re-infection. SARS-CoV-2 Spike protein and its Receptor Binding Area (RBD) are the antigens with larger potential to develop SARS-CoV-2 serological assays. Furthermore, structural research of those antigens are key to know the molecular foundation for Spike interplay with angiotensin changing enzyme 2 receptor, hopefully enabling the event of COVID-19 therapeutics.
Thus, it’s pressing that vital quantities of this protein grew to become accessible on the highest high quality. On this work, we produced Spike and RBD in two human derived cell hosts: HEK293-E6 and Expi293F™. We evaluated the affect of various and scalable bioprocessing approaches on Spike and RBD manufacturing yields and, extra importantly, on these antigens’ high quality attributes. Utilizing destructive and optimistic sera collected from human donors, we present a wonderful efficiency of the produced antigens, assessed in serologic ELISA exams, as denoted by the excessive specificity and sensitivity of the check.
We present sturdy Spike productions with last yields of approx. 2 mg per liter of tradition that have been maintained independently of the manufacturing scale or cell tradition technique. To the most effective of our data, the ultimate yield of 90 mg per liter of tradition obtained for RBD manufacturing, was the best reported so far. An in-depth characterization of SARS-CoV-2 Spike and RBD proteins was carried out, specifically the antigen’s oligomeric state, glycosylation profiles and thermal stability throughout storage.
The correlation of those high quality attributes with ELISA efficiency present equal reactivity to SARS-CoV-2 optimistic serum, for all Spike and RBD produced, and for all storage circumstances examined. Total, we offer simple protocols to provide high-quality SARS-CoV-2 Spike and RBD antigens, that may be simply tailored to each tutorial and industrial settings; and combine, for the primary time, research on the affect of bioprocess with an in-depth characterization of those proteins, correlating antigen’s glycosylation and biophysical attributes to efficiency of COVID-19 serologic exams.
Analysis of IgM LAT and IgM ELISA as in comparison with microscopic agglutination check (MAT) for early analysis of Leptospira sp
Leptospirosis is a worldwide zoonotic illness attributable to spirochetes of the genus Leptospira. The scientific manifestation of leptospirosis is non-specific and continuously misdiagnosed as different sicknesses. The purpose of this research was to match the diagnostic accuracies of two industrial exams for early analysis of Leptospira species: the IgM latex agglutination check (IgM LAT) and the IgM enzyme-linked immunosorbent assay (IgM ELISA).
A complete of 140 serum samples have been obtained from sufferers suspected of leptospirosis on the Universiti Kebangsaan Malaysia Medical Centre (UKMMC). These serum samples have been examined for the presence of Leptospira sp. utilizing IgM LAT, IgM ELISA and MAT. From Desk 1, IgM LAT confirmed 21% (n = 29) optimistic, 18% (n = 25) inconclusive and 61% (n = 86) destructive, whereas IgM ELISA confirmed 6% (n = 8) optimistic, 6% (n = 8) inconclusive, 88% (n = 124) destructive and MAT confirmed 11% (n = 16) optimistic, 47% (n = 65) inconclusive, 42% (n = 59) destructive.
The sensitivity, specificity, optimistic predictive worth (PPV) and destructive predictive worth (NPV) of IgM LAT have been 68.8%, 57.6%, 30.6% and 87.2% respectively, whereas for IgM ELISA they have been 37.5%, 89.8%, 50% and 84.1%, respectively as in comparison with MAT (Desk 2). The outcomes confirmed that IgM LAT had larger sensitivity however decrease specificity in comparison with IgM ELISA.
In conclusion, IgM LAT could be helpful as an early screening check for early analysis of Leptospira sp., whereas IgM ELISA is an acceptable methodology for decreasing false destructive detection of Leptospira sp. As each exams present reasonable percentages (~65%) in accuracy, a further check is required for higher detection of Leptospira sp.
Seroprevalence of Toxoplasma gondii in ruminants through the use of latex agglutination check (LAT) and enzyme-linked immunosorbent assay (ELISA) in Assiut governorate
Toxoplasmosis is a crucial zoonotic parasitic illness world-wide. Nonetheless, knowledge on Toxoplasma gondii an infection in ruminants are scarce in Assiut governorate, Egypt. Thus we carried out a survey on the seroprevalence of T. gondii an infection through the use of latex agglutination check (LAT) and enzyme-linked immunosorbent assay (ELISA). A complete of 274 sera have been examined (56 imported camels, 56 cattle, 55 buffaloes, 50 sheep and 57 goats) within the interval from November 2014 – February 2016.
The general seroprevalence in ruminants was (35.8%) by LAT and (83.6%) by ELISA. The very best charge of T. gondii an infection by LAT was present in goats (47.4%) adopted by sheep (44%), camels (35.7%), cattle (32.1%) and buffaloes (20%). The very best charge of T. gondii an infection by ELISA was recorded in camels (96.4%) adopted by goats (87.7%), sheep (86%), buffaloes (74.5%) and cattle (73.2%). Antibody titres to T. gondii in optimistic sera by LAT ranged from 1:2 to 1:64 in imported camels, 1:2 to 1:32 in cattle, 1:2 to 1:Eight in buffaloes, from 1:Four to 1:64 in sheep and from 1:2 to 1:16 in goats. The variations of seroprevalence between completely different species have been vital by LAT and extremely vital by ELISA. Our outcomes counsel that the T. gondii parasite is extensively unfold on account of excessive publicity to infective cat faeces.
Serological Check to Decide Publicity to SARS-CoV-2: ELISA Based mostly on the Receptor-Binding Area of the Spike Protein (S-RBD N318-V510) Expressed in Escherichia coli
Huge worldwide serological testing for SARS-CoV-2 is required to find out the extent of virus publicity in a selected area, the ratio of symptomatic to asymptomatic contaminated individuals, and the period and extent of immunity after an infection. To realize this, the event and manufacturing of dependable and cost-effective SARS-CoV-2 antigens is vital. We report the bacterial manufacturing of the peptide S-RBDN318-V510, which incorporates the receptor-binding area of the SARS-CoV-2 spike protein (area of 193 amino acid residues from asparagine-318 to valine-510) of the SARS-CoV-2 spike protein.
We purified this peptide utilizing an easy method involving bacterial lysis, his-tag-mediated affinity chromatography, and imidazole-assisted refolding. The antigen performances of S-RBDN318-V510 and a industrial full-length spike protein have been in contrast in ELISAs. In direct ELISAs, the place the antigen was instantly sure to the ELISA floor, each antigens discriminated sera from non-exposed and uncovered people. Nonetheless, the discriminating decision was higher in ELISAs that used the full-spike antigen than the S-RBDN318-V510.
Black 96 Well Plate, 1EA |
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X025-1EA | Arbor Assays | 1EA | EUR 19 |
NanoECM®; 96 Well Plate |
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9601 | Neuromics | each | EUR 38.7 |
Adapter for 96 well plate |
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3510147 | Atto | 1unit | EUR 4422 |
NanoAligned®; 96 Well Plate |
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9602 | Neuromics | each | EUR 41.4 |
Aligned Nanofibers 96 Well Plate |
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3D00012-96 | Neuromics | 700 nm-PCLs | EUR 132 |
Bovine fetuin coated ELISA plates (8x12 wells/plate; 5 plates/pk) |
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800-181-5P | Alpha Diagnostics | 1 pk | EUR 343.2 |
96 Well ELISA Plate, 8-Well, Detachable, High Binding, WhiteFrame & Black Well, |
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504261 | NEST Biotechnology | 50 pcs/cs | EUR 26.29 |
Description: 96 Well ELISA Plate, 8-Well, Detachable, High Binding, WhiteFrame & Black Well, 5/pk, 50/cs |
Streptavidin coated 96-well Plate |
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6523-5 | Biovision | each | EUR 392.4 |
Deep Well Plate 96 Well 2.0 ML - PK50 |
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AXYPDW20C | Scientific Laboratory Supplies | PK50 | EUR 221.4 |
Bovine Collagen Coated 6 Well Plates |
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IBOCLGMH5EA | Innovative research | each | EUR 129 |
Description: Bovine Collagen Coated 6 Well Plates |
Bovine Collagen Coated 48 Well Plates |
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IBOCLGMM5EA | Innovative research | each | EUR 135 |
Description: Bovine Collagen Coated 48 Well Plates |
96 Deep Well Plate Square Well V - PK50 |
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PCR0246 | Scientific Laboratory Supplies | PK50 | EUR 363.15 |
UNIFILTER PLATE 96 WELL 800 UL/WELL - PK25 |
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WHA77002803 | Scientific Laboratory Supplies | PK25 | EUR 1101.6 |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY |
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P-DW-12-HC | CORNING | 5/pk | EUR 182.4 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
96 WELL PCR SEGMENTED PLATE |
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PCR-96-SG-C | CORNING | 10/pk | EUR 174 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & CLear Well, |
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504201 | NEST Biotechnology | 50 pcs/cs | EUR 38.5 |
Description: 96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & CLear Well, 5/pk, 50/cs |
96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & White Well, |
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504271 | NEST Biotechnology | 50 pcs/cs | EUR 51.15 |
Description: 96 Well ELISA Plate, 8-Well, Detachable, High Binding, White Frame & White Well, 5/pk, 50/cs |
Tissue Culture Plate, 96 Well |
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TCP20-96 | Bio Basic | 1 UNIT | EUR 64.18 |
Clear Coated 96 Well Plate, 1EA |
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C107-1EA | Arbor Assays | 1EA | EUR 162 |
Brand 96 Well Deep Well Plate-Stackable - PK24 |
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BR701354-24EA | Scientific Laboratory Supplies | PK24 | EUR 183.6 |
96-Well Protein Folding Plate |
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PFS096 | ProFoldin | kit | EUR 347.84 |
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins. |
BioMag® 96-Well Plate Separator |
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8MB4109S-1 | Polysciences Europe GmbH | 1magnet | EUR 955 |
Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
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PRTA55-5P | Alpha Diagnostics | 1 PK | EUR 315.6 |
Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
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PRTG65-5P | Alpha Diagnostics | 1 PK | EUR 315.6 |
DEEP WELL PLATE 96 WELL 2.0 ML STERIL - PK50 |
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AXYPDW20CS | Scientific Laboratory Supplies | PK50 | EUR 270 |
96 well PCR plate ns - PK50 |
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AXY2164 | Scientific Laboratory Supplies | PK50 | EUR 268.65 |
Polystyrene-bottomed 96-well plate + lid (ELISPOT) |
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CT365 | U-CyTech | 5 | EUR 93.6 |
2 x Clear 96 well Plate, 2EA |
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X003-2EA | Arbor Assays | 2EA | EUR 13 |
5 x Clear 96 well Plate, 5EA |
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X003-5EA | Arbor Assays | 5EA | EUR 25 |
2 x Clear 96 well Plate, 2EA |
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X018-2EA | Arbor Assays | 2EA | EUR 13 |
4 x Clear 96 Well Plate, 4EA |
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X018-4EA | Arbor Assays | 4EA | EUR 31 |
96 Deep Well Plate Round Wells - PK50 |
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PCR0242 | Scientific Laboratory Supplies | PK50 | EUR 329.4 |
Plate 96 Well Immulon II - PK50 |
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3455 | Scientific Laboratory Supplies | PK50 | EUR 318.6 |
96 Well Roche Style PCR Plate |
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PCR1218 | Scientific Laboratory Supplies | PK50 | EUR 223.74 |
Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
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PRTAG75-5P | Alpha Diagnostics | 1 PK | EUR 343.2 |
96 Deep Well Plate Square WellU - PK50 |
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PCR0256 | Scientific Laboratory Supplies | PK50 | EUR 261.9 |
Nunc Lid for 96 well plate - PK50 |
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264122 | Scientific Laboratory Supplies | PK50 | EUR 143.1 |
96 Well Skirted PCR Plate - PK50 |
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PCR1236 | Scientific Laboratory Supplies | PK50 | EUR 433.35 |
96 well filter plate (no filter) |
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SD5006-EMPTY | Bio Basic | 12UNIT | EUR 104.89 |
96 Deep Well Plate Square Wells - PK100 |
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PCR0240 | Scientific Laboratory Supplies | PK100 | EUR 378 |
96 Well Semi Skirted Plate - PK50 |
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PCR1216 | Scientific Laboratory Supplies | PK50 | EUR 229.5 |
Black Half Area 96 Well Plate, 1EA |
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X023-1EA | Arbor Assays | 1EA | EUR 25 |
96 well DNA plate with membrane (960ul each well) |
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SD5007 | Bio Basic | 12UNIT | EUR 291.24 |
96 well RNA plate with membrane (960ul each well) |
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SD5009 | Bio Basic | 12UNIT | EUR 313.16 |
96 Well PCR Plate Clear Wells - PK50 |
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PCR0972 | Scientific Laboratory Supplies | PK50 | EUR 290.25 |
96-well plate Plasmid DNA Prep (mini) |
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TBS6012 | Tribioscience | 2x 96-well Plates | EUR 442 |
Random Access 96 Well Plate Skir - PK50 |
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PCR1412 | Scientific Laboratory Supplies | PK50 | EUR 338.85 |
96-Well Plate Viral DNA Miniprep Kit |
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VT92032 | Bio Basic | 2XPlates, 192prep | EUR 495.86 |
96-Well Plate Plasmid DNA Miniprep Kit |
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BS415 | Bio Basic | 5XPlates, 480prep | EUR 300.37 |
96-Well Plate Plasmid DNA Miniprep Kit |
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BS4152 | Bio Basic | 2XPlates, 192prep | EUR 186.19 |
96-Well Plate DNA Cleanup Miniprep Kit |
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BS369 | Bio Basic | 5XPlates, 480prep | EUR 300.37 |
96-Well Plate DNA Cleanup Miniprep Kit |
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BS3692 | Bio Basic | 2xPlates, 192prep | EUR 186.19 |
96 Well ELISA Plate, Undetachable, High Binding, Clear Frame & CLear Well, 5/pk, |
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514201 | NEST Biotechnology | 50 pcs/cs | EUR 30.36 |
Description: 96 Well ELISA Plate, Undetachable, High Binding, Clear Frame & CLear Well, 5/pk, 50/cs |
PVDF membrane-bottomed 96-well plate + lid (ELISPOT) |
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CT366 | U-CyTech | 5 | EUR 268.8 |
Falcon Microtest Black 96 Well Plate - PK32 |
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353376 | Scientific Laboratory Supplies | PK32 | EUR 244.35 |
96-Well Plate PCR Products Purification Kit |
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BS365 | Bio Basic | 5XPlates, 480prep | EUR 300.37 |
96-Well Plate PCR Products Purification Kit |
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BS3652 | Bio Basic | 2xPlates, 192prep | EUR 186.19 |
Benchmark Beadblaster 96 Adapter for 96 Well Plate - PK2 |
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HOM3208 | Scientific Laboratory Supplies | PK2 | EUR 1923.75 |
96 Well ABI Fast Plate Pcr - PK50 |
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PCR1200 | Scientific Laboratory Supplies | PK50 | EUR 290.25 |
96 WELL CLEAR PCR PLATE FOR ABI |
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PCR-96-AB-C | CORNING | 10/pk | EUR 200.4 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
96 Deep Well Plate Round Wells U - PK100 |
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PCR0228 | Scientific Laboratory Supplies | PK100 | EUR 244.35 |
96 Deep Well Plate Round Wells V - PK100 |
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PCR0230 | Scientific Laboratory Supplies | PK100 | EUR 244.35 |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED |
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P-DW-12-HC-S | CORNING | 5/pk | EUR 248.4 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
96 Well ExoELISA plate (12x8 well strips, pack of 10 plates) |
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EXOEL-96P-1 | SBI | 10 Plates | EUR 255.6 |
Plate 96 Well Immulon 1 FB - PK50 |
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N3355 | Scientific Laboratory Supplies | PK50 | EUR 233.55 |
Nunc Lid for 96 Well Plate SH - PK100 |
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263339 | Scientific Laboratory Supplies | PK100 | EUR 126.9 |
Deep-Multiple Well Plate 96 wells, U ty |
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DM1-50NO | EWC Diagnostics | 1 unit | EUR 483.15 |
Description: Deep-Multiple Well Plate 96 wells, U ty |
Deep-Multiple Well Plate 96 wells, U ty |
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DM4-24NO | EWC Diagnostics | 1 unit | EUR 295.56 |
Description: Deep-Multiple Well Plate 96 wells, U ty |
Deep-Multiple Well Plate 96 wells, U ty |
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DM4-48NO | EWC Diagnostics | 1 unit | EUR 572.66 |
Description: Deep-Multiple Well Plate 96 wells, U ty |
Adapter for 96 well plate, pack of 2 |
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IPD9600-96 | Benchmark Scientific | each | EUR 1337.97 |
96 Well PCR Plate Divisible In 8 |
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PCR1062 | Scientific Laboratory Supplies | PK50 | EUR 154.44 |
Benchmark Beadblaster 96 Eva Cap Mat for 96 Well Deep Well Plate - EACH |
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HOM3232 | Scientific Laboratory Supplies | EACH | EUR 136.35 |
Block for 96 well plate, WSC-2620/30 |
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4002659 | Atto | 1unit | EUR 458.4 |
PCR Plate Non Skirted 96 Well - PK50 |
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MOL2364 | Scientific Laboratory Supplies | PK50 | EUR 348.3 |
96 Well Roche Style Pcr Plate - PK50 |
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PCR1214 | Scientific Laboratory Supplies | PK50 | EUR 330.75 |
PCR Plate Semi Skirted 96 Well - PK50 |
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MOL2366 | Scientific Laboratory Supplies | PK50 | EUR 338.85 |
96 Well Roche Style Qpcr Plate - PK50 |
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PCR1210 | Scientific Laboratory Supplies | PK50 | EUR 318.6 |
Presto™ RNA 96 Well Binding Plate |
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96RBP005 | Geneaid Biotech | each | EUR 132 |
Presto™ RNA 96 Well Binding Plate |
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96RBP100 | Geneaid Biotech | each | EUR 2200 |
96 Well Lung Tumor Genomic DNA Plate |
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D8235152-1 | Biochain | 1 plate | EUR 377 |
96 Well Skirted Plate+ Q-Stick - PK50 |
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PCR1238 | Scientific Laboratory Supplies | PK50 | EUR 311.85 |
Presto™ gDNA 96 Well Binding Plate |
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96GBP005 | Geneaid Biotech | each | EUR 103 |
Presto™ gDNA 96 Well Binding Plate |
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96GBP100 | Geneaid Biotech | each | EUR 1854 |
Presto™ Plasmid 96 Well Filter Plate |
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96PFP005 | Geneaid Biotech | each | EUR 78.5 |
Presto™ Plasmid 96 Well Filter Plate |
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96PFP100 | Geneaid Biotech | each | EUR 1415 |
96 Well Colon Tumor Genomic DNA Plate |
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D8235090-1 | Biochain | 1 plate | EUR 377 |
S100 96-well PCR Plate Adapter - EACH |
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E5391070026 | Scientific Laboratory Supplies | EACH | EUR 426.6 |
S200 96-well PCR Plate Adapter - EACH |
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E5392070038 | Scientific Laboratory Supplies | EACH | EUR 426.6 |
96 Well PCR Plate Divisible In12 - PK50 |
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PCR1064 | Scientific Laboratory Supplies | PK50 | EUR 195.75 |
96 Well Semi-Skirted PCR Plate - PK50 |
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PCR1106 | Scientific Laboratory Supplies | PK50 | EUR 414.45 |
96-Well G-25 Gel Filtration Plate |
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96CG02 | Geneaid Biotech | each | EUR 250 |
96-Well G-25 Gel Filtration Plate |
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96CG04 | Geneaid Biotech | each | EUR 475 |
96-Well G-25 Gel Filtration Plate |
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96CG10 | Geneaid Biotech | each | EUR 1065 |
96-Well G-50 Gel Filtration Plate |
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CGP02 | Geneaid Biotech | each | EUR 250 |
96-Well G-50 Gel Filtration Plate |
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CGP04 | Geneaid Biotech | each | EUR 589.6 |
96-Well G-50 Gel Filtration Plate |
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CGP10 | Geneaid Biotech | each | EUR 1382 |
Presto™ Plasmid 96 Well Binding Plate |
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96PBP005 | Geneaid Biotech | each | EUR 78.5 |
Presto™ Plasmid 96 Well Binding Plate |
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96PBP100 | Geneaid Biotech | each | EUR 1415 |
96 Well Breast Tumor Genomic DNA Plate |
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D8235086-1 | Biochain | 1 plate | EUR 377 |
96 Well Impact MicroBatch Plate, 80/CSE |
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M-673101 | MiTeGen | 80 PLATES | EUR 808 |
Description: 96 Well Impact MicroBatch Plate, 80/CSE |
96 Well Solid Bottom Plate Black - PK100 |
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PCR0354 | Scientific Laboratory Supplies | PK100 | EUR 475.2 |
96 Well Solid Bottom Plate White - PK100 |
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PCR0360 | Scientific Laboratory Supplies | PK100 | EUR 437.4 |
96 WELL PLATE; SQUARE; U BASE; 1.6ML; PP; 96/PK - PK96 |
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60180-P155 | Scientific Laboratory Supplies | PK96 | EUR 631.8 |
Olympus 96-Well PCR Plate, FAST-type |
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24-310 | Genesee Scientific | 25 Plates/Unit | EUR 57.82 |
Description: Low Profile, Natural |
Olympus 96-Well PCR Plate, FAST-type |
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24-310W | Genesee Scientific | 10 Plates/Unit | EUR 29.37 |
Description: Low Profile, White |
96 Well Stomach Tumor Genomic DNA Plate |
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D8235248-1 | Biochain | 1 plate | EUR 377 |
96 Well Plate Blood Genomic DNA Miniprep Kit |
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BT92031 | Bio Basic | 2Plates, 192prep | EUR 210.85 |
96 Well Plate Blood Genomic DNA Miniprep Kit |
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BT92032 | Bio Basic | 5Plates, 480prep | EUR 389.9 |
96 Well Plate Yeast Genomic DNA Miniprep Kit |
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BS8357 | Bio Basic | 2XPlates, 192prep | EUR 234.6 |
Mouse anti-ACTH Clear Coated 96 Well Plate |
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C271-1EA | Arbor Assays | 1EA | EUR 150 |
Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate) |
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M-R-1008649 | MiTeGen | 98 X 1.7 ml | EUR 316 |
Description: Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate) |
96-Well Plate Plant Genomic DNA Miniprep Kit |
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BS8361 | Bio Basic | 2XPlates, 192prep | EUR 234.6 |
CORNING® BIOCOAT™ GELATIN 96 WELL CLEAR FLAT BOTTOM ASSAY PLATE, WITH LID, NONSTERILE, 5/PACK, 50/CASE |
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356689 | CORNING | 5/pk | EUR 800.4 |
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
Olympus 96-Well PCR Plate, Non-Skirted |
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24-300 | Genesee Scientific | 25 Plates/Unit | EUR 57.13 |
Description: Ultra Thin Wall, Natural |
96 Well Plate RNA Cleanup and Concentration Kit |
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BRC1251 | Bio Basic | 2xPlates, 192prep | EUR 300.37 |
96-Well Plate Animal Genomic DNA Miniprep Kit |
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BS437 | Bio Basic | 5XPlates, 480prep | EUR 435.58 |
96-Well Plate Animal Genomic DNA Miniprep Kit |
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BS4372 | Bio Basic | 2XPlates, 192prep | EUR 234.6 |
Olympus 96-Well PCR Plate, Semi-Skirted |
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24-301 | Genesee Scientific | 25 Plates/Unit | EUR 57.82 |
Description: Raised Rim, Natural |
Olympus 96-Well PCR Plate, Full-Skirted |
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24-302 | Genesee Scientific | 25 Plates/Unit | EUR 60.31 |
Description: Ultra Thin Wall, Natural |
Olympus 96-Well PCR Plate, Full-Skirted |
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24-302W | Genesee Scientific | 10 Plates/Unit | EUR 27.75 |
Description: Ultra Thin Wall, White |
Olympus 96-Well PCR Plate, Semi-Skirted |
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27-108 | Genesee Scientific | 10 Plates/Unit | EUR 23.71 |
Description: Flat Rim, Natural |
Olympus 96-Well PCR Plate, Semi-Skirted |
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27-408 | Genesee Scientific | 10 Plates/Unit | EUR 23.76 |
Description: Straight-Sided, Natural |
Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
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M-R-1008647 | MiTeGen | 96 X 1.7 ml | EUR 316 |
Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
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M-R-1008648 | MiTeGen | 97 X 1.7 ml | EUR 316 |
Description: Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
96-well Plasmid DNA Extraction Kit (1 plate) |
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FAPWE-96001 | Favorgen | 1 prep | EUR 175.2 |
96-well Plasmid DNA Extraction Kit (1 plate) |
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FAPWE_96001 | Favorgen | 1 kit | EUR 36 |
96 WELL PLATE; SQUARE; 2ML; PP; 1/PK - EACH |
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60180-P403 | Scientific Laboratory Supplies | EACH | EUR 21.6 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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SK1292 | Bio Basic | 2XPlates, 192prep | EUR 210.85 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
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SK1295 | Bio Basic | 5XPlates, 480prep | EUR 389.9 |
CORNING® BIOCOAT™ T-CELL ACTIVATION CONTROL 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE |
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354730 | CORNING | 5/pk | EUR 102 |
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
2 x Black Half Area 96 Well Plate, 2EA |
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X037-2EA | Arbor Assays | 2EA | EUR 37 |
Bovine IgM ELISA Kit, 96 tests, Quantitative |
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8080 | Alpha Diagnostics | 1 Kit | EUR 854.4 |
EVA Cap Mat for 96 well deep well plate, DNase/RNase Free |
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IPD1196-CM | Benchmark Scientific | each | EUR 92.08 |
96-Well ROCK Activity Assay Kit |
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STA-416 | Cell Biolabs | 96 assays | EUR 750 |
96-Well ROCK Activity Assay Kit |
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STA-416-5 | Cell Biolabs | 5 x 96 assays | EUR 3225 |
PCR Plate 96 Well No Skirt Nat - PK100 |
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LW2200 | Scientific Laboratory Supplies | PK100 | EUR 600.75 |
Olympus 96-well Cuttable PCR Plate, Unskirted |
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24-303C | Genesee Scientific | 10 Plates/Unit | EUR 30.12 |
Description: Polypropylene, Natural |
MULTIWELL PLATE POLYSTYRENE 96-WELL FLA - PK100 |
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M2936-100EA | Scientific Laboratory Supplies | PK100 | EUR 148.35 |
96 Well PCR Plate Divisible In 8 - PK50 |
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PCR1060 | Scientific Laboratory Supplies | PK50 | EUR 195.75 |
UNIFILTER PLATE 96 WELL 800 UL/PER WEL - EACH |
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WHA77002801 | Scientific Laboratory Supplies | EACH | EUR 1098.9 |
Thermo Fast 96 Well PCR Plate - PK25 - PK25 |
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AB0800 | Scientific Laboratory Supplies | PK25 | EUR 286.2 |
NEST MagPure 0.5 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible |
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500021 | NEST Biotechnology | 50 pcs/cs | EUR 31.68 |
Description: NEST MagPure 0.5 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible with Kingfiser Flex&Presto, Non-Sterile, 5/pk, 50/cs |
NEST MagPure 0.5 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible |
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500621 | NEST Biotechnology | 50 pcs/cs | EUR 31.57 |
Description: NEST MagPure 0.5 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible with Kingfiser Flex&Presto, Sterile, 5/pk, 50/cs |
Clear 384 Well Plate |
|||
X105-1EA | Arbor Assays | 1EA | EUR 73 |
96-Well Plate Template Post-It Notes, 4"x6" |
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6290 | Immunochemistry | 100 sheets | EUR 7.8 |
BGMK 6 WELL PLATE |
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53-0606DH | Quidel | each | Ask for price |
Description: flask ; Single or Mixed ; FreshFrozen |
Goat anti-Mouse, Coated White 96 Well Plate, 1EA |
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X010-1EA | Arbor Assays | 1EA | EUR 48 |
96 Well PCR Plates, Fits ABI Fast Plate Machines |
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GP615 | GenDepot | 25 plates | EUR 254.4 |
2'3'-Cyclic GAMP ELISA Kit, (384-well Plate) |
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K067-H1D | Arbor Assays | 1x384 well plate | EUR 1698 |
Description: Direct 2'3'-Cyclic GAMP (cGAMP) ELISA / 384-well plate assay Kit |
Goat anti-Rabbit, Coated White 96 Well Plate, 1EA |
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X014-1EA | Arbor Assays | 1EA | EUR 48 |
Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA |
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X016-1EA | Arbor Assays | 1EA | EUR 48 |
Presto™ PCR Cleanup 96 Well Binding Plate |
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96DBP005 | Geneaid Biotech | each | EUR 72.6 |
Presto™ PCR Cleanup 96 Well Binding Plate |
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96DBP100 | Geneaid Biotech | each | EUR 1306 |
Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA |
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X061-1EA | Arbor Assays | 1EA | EUR 48 |
Donkey anti-Sheep, Coated White 96 Well Plate, 1EA |
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X063-1EA | Arbor Assays | 1EA | EUR 48 |
96-well PCR Plate Non-Skirted (White) - PK25 |
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AB-0600W | Scientific Laboratory Supplies | PK25 | EUR 288.9 |
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
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BS92028 | Bio Basic | 2XPlates, 192prep | EUR 254.7 |
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
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BS92029 | Bio Basic | 5XPlates, 480prep | EUR 437.4 |
96 Deep Well Masterblock Storage Plate, 50/CSE |
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M-780261 | MiTeGen | 50 PLATES | EUR 240 |
Description: 96 Deep Well Masterblock Storage Plate, 50/CSE |
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
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BS585 | Bio Basic | 5XPlates, 480prep | EUR 585.38 |
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
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BS5852 | Bio Basic | 2XPlates, 192prep | EUR 300.37 |
0.5 mL 96 Well Deep Well Plate, V-Bottom, Round Well , Sterile, 10/pk, 50/cs |
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501601 | NEST Biotechnology | 50 pcs/cs | EUR 33.55 |
Description: 0.5 mL 96 Well Deep Well Plate, V-Bottom, Round Well , Sterile, 10/pk, 50/cs |
Olympus 0.1ml 96-Well PCR Plate, Semi-Skirted |
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27-410 | Genesee Scientific | 10 Plates/Unit | EUR 27.23 |
Description: Low Profile, Natural |
96-well PCR Plate Non-Skirted natural - PK25 |
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AB-0600 | Scientific Laboratory Supplies | PK25 | EUR 203.85 |
384 Well Deep Well Plate V - PK100 |
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PCR0316 | Scientific Laboratory Supplies | PK100 | EUR 576.45 |
2.0 mL 96 Well Deep Well Plate, V-Bottom, Square Well, Sterile, 5/pk, 50/cs |
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503501 | NEST Biotechnology | 50 pcs/cs | EUR 49.5 |
Description: 2.0 mL 96 Well Deep Well Plate, V-Bottom, Square Well, Sterile, 5/pk, 50/cs |
Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA |
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C140-1EA | Arbor Assays | 1EA | EUR 150 |
Benchmark Beadblaster 96 96 Well Plate Prefilled with 4mm Beads - PK10 |
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HOM3228 | Scientific Laboratory Supplies | PK10 | EUR 2963.25 |
0.36 mL 96-Well Deep Well Plate, V-Bottom, Round Well, Sterile, 10/pk, 50/cs |
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500161 | NEST Biotechnology | 50 pcs/cs | EUR 54.34 |
Description: 0.36 mL 96-Well Deep Well Plate, V-Bottom, Round Well, Sterile, 10/pk, 50/cs |
0.4 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 10/pk, 50/cs |
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501162 | NEST Biotechnology | 50 pcs/cs | EUR 54.34 |
Description: 0.4 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 10/pk, 50/cs |
1.0 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 5/pk, 50/cs |
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502162 | NEST Biotechnology | 50 pcs/cs | EUR 50.6 |
Description: 1.0 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 5/pk, 50/cs |
2.0 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 5/pk, 50/cs |
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503162 | NEST Biotechnology | 50 pcs/cs | EUR 51.48 |
Description: 2.0 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 5/pk, 50/cs |
1.3 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 5/pk, 50/cs |
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507101 | NEST Biotechnology | 50 pcs/cs | EUR 49.83 |
Description: 1.3 mL 96-Well Deep Well Plate, U-Bottom, Round Well, Sterile, 5/pk, 50/cs |
BioMag® 96-Well Plate Side Pull Magnetic Separator |
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85072S-1 | Polysciences Europe GmbH | 1each | EUR 955 |
INTELLI-PLATE 96-2 Shallow well; 2-well sitting drop, 120 plates |
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MAR-102-0001-20 | MiTeGen | 120 PLATES | EUR 990 |
Description: INTELLI-PLATE 96-2 Shallow well; 2-well sitting drop, 120 plates |
NEST MagPure 2.2 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible w |
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503621 | NEST Biotechnology | 50 pcs/cs | EUR 43.23 |
Description: NEST MagPure 2.2 mL 96-Well Deep Well Plate, V-Bottom, Square Well, Compatible with Kingfiser Flex&Presto, Sterile, 5/pk, 50/cs |
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Attachment of the antigens to the ELISA floor utilizing a layer of anti-histidine antibodies gave equal decision for each S-RBDN318-V510 and the full-length spike protein. Outcomes exhibit that ELISA-functional SARS-CoV-2 antigens could be produced in bacterial cultures, and that S-RBDN318-V510 could symbolize a cheap different to using structurally extra advanced antigens in serological COVID-19 testing.
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