Production of high-quality SARS-CoV-2 antigens: impact of bioprocess and storage on glycosylation, biophysical attributes, and ELISA serologic tests performance
Serological assays are helpful instruments to check SARS-CoV-2 unfold and, importantly, to determine people that have been already contaminated and could be probably resistant to a virus re-infection. SARS-CoV-2 Spike protein and its Receptor Binding Area (RBD) are the antigens with larger potential to develop SARS-CoV-2 serological assays. Furthermore, structural research of those antigens are key to know the molecular foundation for Spike interplay with angiotensin changing enzyme 2 receptor, hopefully enabling the event of COVID-19 therapeutics.
Thus, it’s pressing that vital quantities of this protein grew to become accessible on the highest high quality. On this work, we produced Spike and RBD in two human derived cell hosts: HEK293-E6 and Expi293F™. We evaluated the affect of various and scalable bioprocessing approaches on Spike and RBD manufacturing yields and, extra importantly, on these antigens’ high quality attributes. Utilizing destructive and optimistic sera collected from human donors, we present a wonderful efficiency of the produced antigens, assessed in serologic ELISA exams, as denoted by the excessive specificity and sensitivity of the check.
We present sturdy Spike productions with last yields of approx. 2 mg per liter of tradition that have been maintained independently of the manufacturing scale or cell tradition technique. To the most effective of our data, the ultimate yield of 90 mg per liter of tradition obtained for RBD manufacturing, was the best reported so far. An in-depth characterization of SARS-CoV-2 Spike and RBD proteins was carried out, specifically the antigen’s oligomeric state, glycosylation profiles and thermal stability throughout storage.
The correlation of those high quality attributes with ELISA efficiency present equal reactivity to SARS-CoV-2 optimistic serum, for all Spike and RBD produced, and for all storage circumstances examined. Total, we offer simple protocols to provide high-quality SARS-CoV-2 Spike and RBD antigens, that may be simply tailored to each tutorial and industrial settings; and combine, for the primary time, research on the affect of bioprocess with an in-depth characterization of those proteins, correlating antigen’s glycosylation and biophysical attributes to efficiency of COVID-19 serologic exams.
Analysis of IgM LAT and IgM ELISA as in comparison with microscopic agglutination check (MAT) for early analysis of Leptospira sp
Leptospirosis is a worldwide zoonotic illness attributable to spirochetes of the genus Leptospira. The scientific manifestation of leptospirosis is non-specific and continuously misdiagnosed as different sicknesses. The purpose of this research was to match the diagnostic accuracies of two industrial exams for early analysis of Leptospira species: the IgM latex agglutination check (IgM LAT) and the IgM enzyme-linked immunosorbent assay (IgM ELISA).
A complete of 140 serum samples have been obtained from sufferers suspected of leptospirosis on the Universiti Kebangsaan Malaysia Medical Centre (UKMMC). These serum samples have been examined for the presence of Leptospira sp. utilizing IgM LAT, IgM ELISA and MAT. From Desk 1, IgM LAT confirmed 21% (n = 29) optimistic, 18% (n = 25) inconclusive and 61% (n = 86) destructive, whereas IgM ELISA confirmed 6% (n = 8) optimistic, 6% (n = 8) inconclusive, 88% (n = 124) destructive and MAT confirmed 11% (n = 16) optimistic, 47% (n = 65) inconclusive, 42% (n = 59) destructive.
The sensitivity, specificity, optimistic predictive worth (PPV) and destructive predictive worth (NPV) of IgM LAT have been 68.8%, 57.6%, 30.6% and 87.2% respectively, whereas for IgM ELISA they have been 37.5%, 89.8%, 50% and 84.1%, respectively as in comparison with MAT (Desk 2). The outcomes confirmed that IgM LAT had larger sensitivity however decrease specificity in comparison with IgM ELISA.
In conclusion, IgM LAT could be helpful as an early screening check for early analysis of Leptospira sp., whereas IgM ELISA is an acceptable methodology for decreasing false destructive detection of Leptospira sp. As each exams present reasonable percentages (~65%) in accuracy, a further check is required for higher detection of Leptospira sp.

Seroprevalence of Toxoplasma gondii in ruminants through the use of latex agglutination check (LAT) and enzyme-linked immunosorbent assay (ELISA) in Assiut governorate
Toxoplasmosis is a crucial zoonotic parasitic illness world-wide. Nonetheless, knowledge on Toxoplasma gondii an infection in ruminants are scarce in Assiut governorate, Egypt. Thus we carried out a survey on the seroprevalence of T. gondii an infection through the use of latex agglutination check (LAT) and enzyme-linked immunosorbent assay (ELISA). A complete of 274 sera have been examined (56 imported camels, 56 cattle, 55 buffaloes, 50 sheep and 57 goats) within the interval from November 2014 – February 2016.
The general seroprevalence in ruminants was (35.8%) by LAT and (83.6%) by ELISA. The very best charge of T. gondii an infection by LAT was present in goats (47.4%) adopted by sheep (44%), camels (35.7%), cattle (32.1%) and buffaloes (20%). The very best charge of T. gondii an infection by ELISA was recorded in camels (96.4%) adopted by goats (87.7%), sheep (86%), buffaloes (74.5%) and cattle (73.2%). Antibody titres to T. gondii in optimistic sera by LAT ranged from 1:2 to 1:64 in imported camels, 1:2 to 1:32 in cattle, 1:2 to 1:Eight in buffaloes, from 1:Four to 1:64 in sheep and from 1:2 to 1:16 in goats. The variations of seroprevalence between completely different species have been vital by LAT and extremely vital by ELISA. Our outcomes counsel that the T. gondii parasite is extensively unfold on account of excessive publicity to infective cat faeces.
Serological Check to Decide Publicity to SARS-CoV-2: ELISA Based mostly on the Receptor-Binding Area of the Spike Protein (S-RBD N318-V510) Expressed in Escherichia coli
Huge worldwide serological testing for SARS-CoV-2 is required to find out the extent of virus publicity in a selected area, the ratio of symptomatic to asymptomatic contaminated individuals, and the period and extent of immunity after an infection. To realize this, the event and manufacturing of dependable and cost-effective SARS-CoV-2 antigens is vital. We report the bacterial manufacturing of the peptide S-RBDN318-V510, which incorporates the receptor-binding area of the SARS-CoV-2 spike protein (area of 193 amino acid residues from asparagine-318 to valine-510) of the SARS-CoV-2 spike protein.
We purified this peptide utilizing an easy method involving bacterial lysis, his-tag-mediated affinity chromatography, and imidazole-assisted refolding. The antigen performances of S-RBDN318-V510 and a industrial full-length spike protein have been in contrast in ELISAs. In direct ELISAs, the place the antigen was instantly sure to the ELISA floor, each antigens discriminated sera from non-exposed and uncovered people. Nonetheless, the discriminating decision was higher in ELISAs that used the full-spike antigen than the S-RBDN318-V510.
Adapter for 96 well plate |
3510147 |
Atto |
1unit |
EUR 6697.2 |
Bovine fetuin coated ELISA plates (8x12 wells/plate; 5 plates/pk) |
800-181-5P |
Alpha Diagnostics |
1 pk |
EUR 343.2 |
Aligned Nanofibers 96 Well Plate |
3D00012-96 |
Neuromics |
700 nm-PCLs |
EUR 132 |
Streptavidin coated 96-well Plate |
6523-5 |
Biovision |
each |
EUR 392.4 |
96 WELL PCR SEGMENTED PLATE |
PCR-96-SG-C |
CORNING |
10/pk |
EUR 174 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY |
P-DW-12-HC |
CORNING |
5/pk |
EUR 182.4 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
Tissue Culture Plate, 96 Well |
TCP20-96 |
Bio Basic |
1 UNIT |
EUR 64.18 |
|
Clear Coated 96 Well Plate, 1EA |
C107-1EA |
Arbor Assays |
1EA |
EUR 162 |
96-Well Protein Folding Plate |
PFS096 |
ProFoldin |
kit |
EUR 347.84 |
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins. |
Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
PRTA55-5P |
Alpha Diagnostics |
1 PK |
EUR 315.6 |
Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
PRTG65-5P |
Alpha Diagnostics |
1 PK |
EUR 315.6 |
2 x Clear 96 well Plate, 2EA |
X003-2EA |
Arbor Assays |
2EA |
EUR 13 |
5 x Clear 96 well Plate, 5EA |
X003-5EA |
Arbor Assays |
5EA |
EUR 25 |
2 x Clear 96 well Plate, 2EA |
X018-2EA |
Arbor Assays |
2EA |
EUR 13 |
4 x Clear 96 Well Plate, 4EA |
X018-4EA |
Arbor Assays |
4EA |
EUR 31 |
Polystyrene-bottomed 96-well plate + lid (ELISPOT) |
CT365 |
U-CyTech |
5 |
EUR 93.6 |
96 well filter plate (no filter) |
SD5006-EMPTY |
Bio Basic |
12UNIT |
EUR 104.89 |
|
Black Half Area 96 Well Plate, 1EA |
X023-1EA |
Arbor Assays |
1EA |
EUR 25 |
Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack |
PRTAG75-5P |
Alpha Diagnostics |
1 PK |
EUR 343.2 |
96 well DNA plate with membrane (960ul each well) |
SD5007 |
Bio Basic |
12UNIT |
EUR 291.24 |
|
96 well RNA plate with membrane (960ul each well) |
SD5009 |
Bio Basic |
12UNIT |
EUR 313.16 |
|
96-Well Plate Viral DNA Miniprep Kit |
VT92032 |
Bio Basic |
2XPlates, 192prep |
EUR 495.86 |
|
96-Well Plate Plasmid DNA Miniprep Kit |
BS415 |
Bio Basic |
5XPlates, 480prep |
EUR 300.37 |
|
96-Well Plate Plasmid DNA Miniprep Kit |
BS4152 |
Bio Basic |
2XPlates, 192prep |
EUR 186.19 |
|
96-Well Plate DNA Cleanup Miniprep Kit |
BS369 |
Bio Basic |
5XPlates, 480prep |
EUR 300.37 |
|
96-Well Plate DNA Cleanup Miniprep Kit |
BS3692 |
Bio Basic |
2xPlates, 192prep |
EUR 186.19 |
|
PVDF membrane-bottomed 96-well plate + lid (ELISPOT) |
CT366 |
U-CyTech |
5 |
EUR 268.8 |
96 WELL CLEAR PCR PLATE FOR ABI |
PCR-96-AB-C |
CORNING |
10/pk |
EUR 200.4 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
96-Well Plate PCR Products Purification Kit |
BS365 |
Bio Basic |
5XPlates, 480prep |
EUR 300.37 |
|
96-Well Plate PCR Products Purification Kit |
BS3652 |
Bio Basic |
2xPlates, 192prep |
EUR 186.19 |
|
96-Well ROCK Activity Assay Kit |
STA-416 |
Cell Biolabs |
96 assays |
EUR 1027.2 |
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
96-Well ROCK Activity Assay Kit |
STA-416-5 |
Cell Biolabs |
5 x 96 assays |
EUR 4228.8 |
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1. |
96 Well ExoELISA plate (12x8 well strips, pack of 10 plates) |
EXOEL-96P-1 |
SBI |
10 Plates |
EUR 255.6 |
1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED |
P-DW-12-HC-S |
CORNING |
5/pk |
EUR 248.4 |
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen |
Block for 96 well plate, WSC-2620/30 |
4002659 |
Atto |
1unit |
EUR 458.4 |
Description: Bl ock for WSC-2620 / 2630 |
OxiSelect 96-Well Comet Assay Kit |
STA-355 |
Cell Biolabs |
96 assays |
EUR 512.4 |
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
OxiSelect 96-Well Comet Assay Kit |
STA-355-5 |
Cell Biolabs |
5 x 96 assays |
EUR 1959.6 |
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope. |
CORNING® BIOCOAT™ GELATIN 96 WELL CLEAR FLAT BOTTOM ASSAY PLATE, WITH LID, NONSTERILE, 5/PACK, 50/CASE |
356689 |
CORNING |
5/pk |
EUR 800.4 |
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
96 Well Lung Tumor Genomic DNA Plate |
D8235152-1 |
Biochain |
1 plate |
EUR 328 |
Radius 96-Well Cell Migration Assay |
CBA-126 |
Cell Biolabs |
96 assays |
EUR 686.4 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 96-Well Cell Migration Assay |
CBA-126-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2697.6 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
OxiSelect 96-Well Comet Assay Slide |
STA-356 |
Cell Biolabs |
1 slide |
EUR 219.6 |
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
OxiSelect 96-Well Comet Assay Slide |
STA-356-5 |
Cell Biolabs |
5 slides |
EUR 622.8 |
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy. |
96 Well Colon Tumor Genomic DNA Plate |
D8235090-1 |
Biochain |
1 plate |
EUR 328 |
96 Well Breast Tumor Genomic DNA Plate |
D8235086-1 |
Biochain |
1 plate |
EUR 328 |
96 Well Impact MicroBatch Plate, 80/CSE |
M-673101 |
MiTeGen |
80 PLATES |
EUR 808 |
Description: 96 Well Impact MicroBatch Plate, 80/CSE |
96 Well Stomach Tumor Genomic DNA Plate |
D8235248-1 |
Biochain |
1 plate |
EUR 328 |
96 Well Plate Blood Genomic DNA Miniprep Kit |
BT92031 |
Bio Basic |
2Plates, 192prep |
EUR 210.85 |
|
96 Well Plate Blood Genomic DNA Miniprep Kit |
BT92032 |
Bio Basic |
5Plates, 480prep |
EUR 389.9 |
|
Mouse anti-ACTH Clear Coated 96 Well Plate |
C271-1EA |
Arbor Assays |
1EA |
EUR 150 |
96 Well Plate Yeast Genomic DNA Miniprep Kit |
BS8357 |
Bio Basic |
2XPlates, 192prep |
EUR 234.6 |
|
CORNING® BIOCOAT™ T-CELL ACTIVATION CONTROL 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE |
354730 |
CORNING |
5/pk |
EUR 102 |
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
96-Well Plate Plant Genomic DNA Miniprep Kit |
BS8361 |
Bio Basic |
2XPlates, 192prep |
EUR 234.6 |
|
Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate) |
M-R-1008649 |
MiTeGen |
98 X 1.7 ml |
EUR 316 |
Description: Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate) |
96 Well Plate RNA Cleanup and Concentration Kit |
BRC1251 |
Bio Basic |
2xPlates, 192prep |
EUR 300.37 |
|
96-Well Plate Animal Genomic DNA Miniprep Kit |
BS437 |
Bio Basic |
5XPlates, 480prep |
EUR 435.58 |
|
96-Well Plate Animal Genomic DNA Miniprep Kit |
BS4372 |
Bio Basic |
2XPlates, 192prep |
EUR 234.6 |
|
96-well Plasmid DNA Extraction Kit (1 plate) |
FAPWE-96001 |
Favorgen |
1 prep |
EUR 175.2 |
2 x Black Half Area 96 Well Plate, 2EA |
X037-2EA |
Arbor Assays |
2EA |
EUR 37 |
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
SK1292 |
Bio Basic |
2XPlates, 192prep |
EUR 210.85 |
|
96-Well Plate Bacteria Genomic DNA Miniprep Kit |
SK1295 |
Bio Basic |
5XPlates, 480prep |
EUR 389.9 |
|
Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
M-R-1008647 |
MiTeGen |
96 X 1.7 ml |
EUR 316 |
Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
M-R-1008648 |
MiTeGen |
97 X 1.7 ml |
EUR 316 |
Description: Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate) |
CytoSelect 96-well Cell Transformation Assay |
CBA-130 |
Cell Biolabs |
96 assays |
EUR 866.4 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Cell Transformation Assay |
CBA-130-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3463.2 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Phagocytosis Assay (Zymosan) |
CBA-224 |
Cell Biolabs |
96 assays |
EUR 866.4 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
CytoSelect 96-well Phagocytosis Assay (Zymosan) |
CBA-224-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3525.6 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
BCA Protein Assay Ready to use pre-coated bovine gamma globulin (BGG) plate 0-20 µg, 5 plates/pk, for use with Enhanced BCA Kit |
PBGG-5 |
Alpha Diagnostics |
1 pk |
EUR 270 |
BCA Protein Assay Ready to use pre-coated bovine serum albumin (BSA) plate 0-20 µg, 5 plates/pk for use with Enhanced BCA Kit |
PBSA-5 |
Alpha Diagnostics |
1 pk |
EUR 270 |
96 Well PCR Plates, Fits ABI Fast Plate Machines |
GP615 |
GenDepot |
25 plates |
EUR 254.4 |
Goat anti-Mouse, Coated White 96 Well Plate, 1EA |
X010-1EA |
Arbor Assays |
1EA |
EUR 48 |
2'3'-Cyclic GAMP ELISA Kit, (384-well Plate) |
K067-H1D |
Arbor Assays |
1x384 well plate |
EUR 1557 |
Goat anti-Rabbit, Coated White 96 Well Plate, 1EA |
X014-1EA |
Arbor Assays |
1EA |
EUR 48 |
Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA |
X016-1EA |
Arbor Assays |
1EA |
EUR 48 |
Bovine IgM ELISA kit |
55R-1928 |
Fitzgerald |
96 tests |
EUR 708 |
Description: ELISA Kit for detection of IgM in the research laboratory |
Bovine IgM ELISA Kit |
EBI0045 |
Abclonal |
96Tests |
EUR 625.2 |
Bovine IgM ELISA Kit |
EBI0104 |
Abclonal |
96Tests |
EUR 625.2 |
Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA |
X061-1EA |
Arbor Assays |
1EA |
EUR 48 |
Donkey anti-Sheep, Coated White 96 Well Plate, 1EA |
X063-1EA |
Arbor Assays |
1EA |
EUR 48 |
96 Deep Well Masterblock Storage Plate, 50/CSE |
M-780261 |
MiTeGen |
50 PLATES |
EUR 240 |
Description: 96 Deep Well Masterblock Storage Plate, 50/CSE |
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
BS92028 |
Bio Basic |
2XPlates, 192prep |
EUR 254.7 |
|
Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit |
BS92029 |
Bio Basic |
5XPlates, 480prep |
EUR 437.4 |
|
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
BS585 |
Bio Basic |
5XPlates, 480prep |
EUR 585.38 |
|
96 Well Plate Bacterial Total RNA Miniprep Super Kit |
BS5852 |
Bio Basic |
2XPlates, 192prep |
EUR 300.37 |
|
96-well Checkpoint Kinase Activity Assay Kit |
STA-414 |
Cell Biolabs |
96 assays |
EUR 908.4 |
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
96-well Checkpoint Kinase Activity Assay Kit |
STA-414-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3672 |
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors. |
CORNING® BIOCOAT™ T-CELL ACTIVATION ANTI-MOUSE CD3 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE |
354720 |
CORNING |
5/pk |
EUR 576 |
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
CORNING® BIOCOAT™ T-CELL ACTIVATION ANTI-HUMAN CD3 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE |
354725 |
CORNING |
5/pk |
EUR 568.8 |
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL |
Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA |
C140-1EA |
Arbor Assays |
1EA |
EUR 150 |
384 Well Plate, 10/Bag |
PCR-384-SN |
Bio Basic |
1PK, 10UNIT |
EUR 112.2 |
|
96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE |
PCR-96-HS-C |
CORNING |
10/pk |
EUR 190.8 |
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA |
C075-1EA |
Arbor Assays |
1EA |
EUR 150 |
Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA |
X011-1EA |
Arbor Assays |
1EA |
EUR 48 |
INTELLI-PLATE 96-2 Shallow well; 2-well sitting drop, 120 plates |
MAR-102-0001-20 |
MiTeGen |
120 PLATES |
EUR 990 |
Description: INTELLI-PLATE 96-2 Shallow well; 2-well sitting drop, 120 plates |
Modular In Situ-1 96 Well Crystallization Plate |
ML-MIS-FP3D |
MiTeGen |
1 PLATE |
EUR 392 |
Description: Modular In Situ-1 96 Well Crystallization Plate |
CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays) |
CBA-320 |
Cell Biolabs |
96 assays |
EUR 644.4 |
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis. |
Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA |
C223-1EA |
Arbor Assays |
1EA |
EUR 150 |
Attachment of the antigens to the ELISA floor utilizing a layer of anti-histidine antibodies gave equal decision for each S-RBDN318-V510 and the full-length spike protein. Outcomes exhibit that ELISA-functional SARS-CoV-2 antigens could be produced in bacterial cultures, and that S-RBDN318-V510 could symbolize a cheap different to using structurally extra advanced antigens in serological COVID-19 testing.