Diagnostic accuracy of Fusobacterium nucleatum IgA and IgG ELISA test in colorectal cancer
July 23, 2021
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The colorectal most cancers is a severe well being drawback. The analysis of the illness largely depends on an invasive process. A non-invasive diagnostic check similar to an immunoassay, might facilitate analysis of colorectal most cancers. The aim of the research was to judge the usage of antibodies towards Fusobacterium nucleatum within the analysis of colorectal most cancers (CRC).
Completely 78 sufferers in three teams had been included within the research. F. nucleatum within the tissues was detected utilizing quantitative polymerase chain response assay. F. nucleatum IgA and IgG had been measured utilizing enzyme linked immunosorbent assay. F. nucleatum was detected in 86.7% and 73.1% circumstances of CRC and precancerous-benign colon illness (P-BCD), respectively. The OD values from F. nucleatum IgA and IgG ELISA assessments had been increased in CRC group in contrast with wholesome people.
The sensitivity of IgA ELISA check different between 31.eight and 95.5% relying on the chosen cut-off values. The positivity charge of antibodies in sufferers with excessive quantity of F. nucleatum in tissue was considerably larger than within the adverse group. The F. nucleatum IgA and IgG antibodies in CRC had been increased than those in wholesome controls however the discriminative capacity of the ELISA check was not sufficient to be thought-about as a diagnostic instrument.
Analyzing Gluten Content material in Numerous Meals Merchandise Utilizing Totally different Varieties of ELISA Check Kits
Gluten is an insoluble protein produced when glutelins and prolamins, that are present in grains similar to wheat, barley, and oats, mix to type an elastic skinny movie. This dietary gluten may cause extreme contraction of the intestinal mucous membrane in some individuals, stopping nutrient absorption. This situation, referred to as celiac illness (CD), impacts roughly 1% of the world’s inhabitants. The one present remedy for sufferers with CD and comparable ailments is lifelong avoidance of gluten.
To research the gluten content material in meals, varied enzyme-linked immunosorbent assay (ELISA) assessments are at present used. On this research, the gluten content material in varied meals merchandise was analyzed utilizing completely different sorts of ELISA check kits. For gluten-free meals, three completely different ELISA check kits largely yielded values under the restrict of detection.
Nevertheless, gluten was detected at 24.0-40.2 g/kg in bread, 6.5-72.6 g/kg in noodles, and 23.0-86.9 g/kg in several powder meals samples. A big distinction (p < 0.05) in gluten content material was noticed for these gluten-containing meals merchandise. Reproducibility points recommend that it’s essential to make use of a number of ELISA kits for the correct detection and quantification of gluten in varied meals merchandise reasonably than utilizing one ELISA equipment.
Diagnostic accuracy of direct agglutination check, rK39 ELISA and 6 speedy diagnostic assessments amongst visceral leishmaniasis sufferers with and with out HIV coinfection in Ethiopia
Analysis of a first-time visceral leishmaniasis (VL) an infection in Ethiopia is established by use of a speedy diagnostic check (RDT) detecting antibodies towards rK39, direct agglutination check (DAT) and microscopy in response to the nationwide algorithm. The efficiency of particular person assessments and algorithm is variable and is determined by a number of components, one being HIV standing. Restricted information can be found on the efficiency of assessments in VL-HIV coinfected sufferers.
Evaluation of the efficiency of DAT (ITM-A), rK39 ELISA (Serion) and 6 RDT (Onsite Leishmania Ab CTK, Antigen ICT Xinjier, IT Leish Biorad, Kalazar Detect Inbios, rK39 IgG1 Coris, rk28 IgG1 Coris) for the analysis of VL was carried out on a panel of 91 saved serum and plasma samples of ‘first-episode’ suspected VL sufferers, with HIV coinfection (n = 51) and with out (n = 40). A mixed reference normal was used: both optimistic microscopy on tissue aspirates, or in case of adverse microscopy, optimistic PCR outcomes on the aspirate slide.
Moreover, endemic wholesome controls (n = 20), non-endemic controls (n = 10) and sufferers with confirmed malaria an infection (n = 10) had been examined for specificity analysis. Sensitivities ranged from 69.2% for DAT (utilized cut-off ≥ 1/3200) to 92.2% for the Onsite RDT, whereas specificities ranged from 20.0% for Kalazar Antigen ICT to 100% for IT Leish and rK39 IgG1. Sensitivities from all assays decreased upon stratification in response to HIV standing however was solely considerably completely different for rK39 Serion ELISA (p-value 0.0084) and the Onsite RDT (p-value 0.0159).
In conclusion, efficiency of commercially obtainable assays for VL on samples from Northern-Ethiopian sufferers different extensively with a considerable lower in sensitivity within the VL-HIV coinfected group. Clear pointers on minimal efficiency standards of particular person assessments and algorithms are wanted, in addition to which reference normal needs to be used to find out the efficiency.
Validation of a Novel Industrial ELISA Check for the Detection of Antibodies towards Coxiella burnetii
Q fever is a zoonosis brought on by Coxiella burnetii, a Gram-negative pathogen with a posh life cycle and a excessive affect on public and animal well being everywhere in the world. The signs are indistinguishable from these belonging to different ailments, and the illness might be symptomless. For these causes, dependable laboratory assessments are important for an correct analysis.
The intention of this research was to validate a novel enzyme-linked immunosorbent assay (ELISA) check, named the Refrain Q Fever Section II IgG and IgM Package (DIESSE, Diagnostica Senese S.p.A), which is carried out by an instrument named Refrain, a brand new machine in medical diagnostics. This diagnostic check is employed for the detection of antibodies towards C. burnetii Section II antigens in acute illness. Our validation protocol was carried out in response to the Italian Accreditation Physique (ACCREDIA) (Regulation UNI CEI EN ISO/IEC 17025:2018 and 17043:2010), OIE (World Group for Animal Well being), and Assertion for Reporting Research of Diagnostic Accuracy (STARD).
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Description: Canine (Canis familiaris; Dog) |
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Canine Plasminogen (PLG) ELISA Kit |
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| AE26985DO-48Tests | Abebio | 48 Tests | EUR 360 |
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Description: Canine (Canis familiaris; Dog) |
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| AE26985DO-96T | Abebio | 96T | EUR 680 |
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Description: Canine (Canis familiaris; Dog) |
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Canine Plasminogen (PLG) ELISA Kit |
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| AE26985DO-96Tests | Abebio | 96 Tests | EUR 680 |
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Description: Canine (Canis familiaris; Dog) |
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Description: Sandwich ELISA |
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Canine Plasminogen (PLG) ELISA Kit |
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Canine PLG(Plasminogen) ELISA Kit |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Canine;Sensitivity: 0.094 ng/ml |
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| E01A68167 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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ELISA kit for Canine Plasminogen (PLG) |
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| KTE20045-48T | Abbkine | 48T | EUR 424.8 |
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Description: Quantitative sandwich ELISA for measuring Canine Plasminogen (PLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Canine Plasminogen (PLG) |
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| KTE20045-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2702.4 |
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Description: Quantitative sandwich ELISA for measuring Canine Plasminogen (PLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Canine Plasminogen (PLG) |
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| KTE20045-96T | Abbkine | 96T | EUR 686.4 |
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Description: Quantitative sandwich ELISA for measuring Canine Plasminogen (PLG) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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Nori® Canine Plasminogen ELISA Kit |
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| GR115306 | Genorise Scientific | 96-well | EUR 461 |
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Mouse plasminogen total antigen ELISA assay |
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| MBS088023-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
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Human plasminogen total antigen assay ELISA Kit |
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| E01A67916 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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| E01A67942 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Canine Plasminogen Activator Inhibitor 1 ELISA kit |
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| E08P0028-48wellsplate | BlueGene | 48 wells plate | EUR 280 |
Canine Plasminogen Activator Inhibitor 1 ELISA kit |
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| E08P0028-96wellsplate | BlueGene | 96 wells plate | EUR 405 |
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| E08P0057-96wellsplate | BlueGene | 96 wells plate | EUR 405 |
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| MBS037967-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
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| MBS037967-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
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| MBS037967-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Canine Plasminogen Activator Inhibitor 1 ELISA Kit |
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| MBS037967-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
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| MBS024905-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Canine Plasminogen Activator Inhibitor 2 ELISA Kit |
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| MBS024905-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Canine Plasminogen Activator Inhibitor 2 ELISA Kit |
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| MBS024905-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Canine Plasminogen Activator Inhibitor 2 ELISA Kit |
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| MBS024905-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Canine Plasminogen Activator Inhibitor 2 ELISA Kit |
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| MBS734435-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 5685 |
Canine Plasminogen Activator Inhibitor 2 ELISA Kit |
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| MBS734435-48StripWells | MyBiosource | 48-Strip-Wells | EUR 485 |
Canine Plasminogen Activator Inhibitor 2 ELISA Kit |
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| MBS734435-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3020 |
Canine Plasminogen Activator Inhibitor 2 ELISA Kit |
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| MBS734435-96StripWells | MyBiosource | 96-Strip-Wells | EUR 690 |
Canine Plasminogen Activator Inhibitor 1 ELISA Kit |
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| MBS743848-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 5685 |
Canine Plasminogen Activator Inhibitor 1 ELISA Kit |
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| MBS743848-48StripWells | MyBiosource | 48-Strip-Wells | EUR 485 |
Canine Plasminogen Activator Inhibitor 1 ELISA Kit |
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| MBS743848-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3020 |
Canine Plasminogen Activator Inhibitor 1 ELISA Kit |
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| MBS743848-96StripWells | MyBiosource | 96-Strip-Wells | EUR 690 |
Canine Urokinase plasminogen activator ELISA kit |
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| E08U0012-48wellsplate | BlueGene | 48 wells plate | EUR 280 |
Canine Urokinase plasminogen activator ELISA kit |
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| E08U0012-96wellsplate | BlueGene | 96 wells plate | EUR 405 |
Canine Urokinase plasminogen activator ELISA kit |
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| E01A69595 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Canine Urokinase Plasminogen Activator ELISA Kit |
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| MBS020636-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Canine Urokinase Plasminogen Activator ELISA Kit |
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| MBS020636-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Canine Urokinase Plasminogen Activator ELISA Kit |
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| MBS020636-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Canine Urokinase Plasminogen Activator ELISA Kit |
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| MBS020636-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Canine Urokinase plasminogen activator ELISA Kit |
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| MBS736077-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 5685 |
Canine Urokinase plasminogen activator ELISA Kit |
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| MBS736077-48StripWells | MyBiosource | 48-Strip-Wells | EUR 485 |
Canine Urokinase plasminogen activator ELISA Kit |
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| MBS736077-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3020 |
Canine Urokinase plasminogen activator ELISA Kit |
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| MBS736077-96StripWells | MyBiosource | 96-Strip-Wells | EUR 690 |
Canine Plasminogen Activator Inhibitor (PAI) ELISA Kit |
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| EIA06167Ca | Enlibio | each | EUR 520 |
Canine Plasminogen Activator Inhibitor (PAI) ELISA Kit |
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| MBS2603087-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3545 |
Canine Plasminogen Activator Inhibitor (PAI) ELISA Kit |
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| MBS2603087-48StripWells | MyBiosource | 48-Strip-Wells | EUR 310 |
Canine Plasminogen Activator Inhibitor (PAI) ELISA Kit |
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| MBS2603087-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1940 |
Canine Plasminogen Activator Inhibitor (PAI) ELISA Kit |
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| MBS2603087-96StripWells | MyBiosource | 96-Strip-Wells | EUR 475 |
Canine Plasminogen Activator Inhibitor(PAI)ELISA Kit |
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| NSL1963Ca | Sunlong | 2058 wells | EUR 528 |
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Description: Sandwich ELISA |
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Canine Plasminogen Activator, Tissue (t-PA) ELISA KIT |
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| EIA06466Ca | Enlibio | each | EUR 520 |
Canine Plasminogen Activator, Tissue (t-PA) ELISA Kit |
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| MBS2608321-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3545 |
Canine Plasminogen Activator, Tissue (t-PA) ELISA Kit |
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| MBS2608321-48StripWells | MyBiosource | 48-Strip-Wells | EUR 310 |
Canine Plasminogen Activator, Tissue (t-PA) ELISA Kit |
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| MBS2608321-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1940 |
Canine Plasminogen Activator, Tissue (t-PA) ELISA Kit |
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| MBS2608321-96StripWells | MyBiosource | 96-Strip-Wells | EUR 475 |
Canine Plasminogen Activator,Tissue(t-PA) ELISA Kit |
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| NSL0991Ca | Sunlong | 1086 wells | EUR 528 |
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Description: Sandwich ELISA |
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Canine Plasminogen-Related Protein B (PRPB) ELISA Kit |
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| MBS9393656-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Canine Plasminogen-Related Protein B (PRPB) ELISA Kit |
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| MBS9393656-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Canine Plasminogen-Related Protein B (PRPB) ELISA Kit |
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| MBS9393656-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Canine Plasminogen-Related Protein B (PRPB) ELISA Kit |
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| MBS9393656-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Canine Tissue type Plasminogen Activator ELISA kit |
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| E01A69178 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Canine Tissue type Plasminogen Activator ELISA kit |
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| E08T0010-48wellsplate | BlueGene | 48 wells plate | EUR 280 |
Canine Tissue type Plasminogen Activator ELISA kit |
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| E08T0010-96wellsplate | BlueGene | 96 wells plate | EUR 405 |
Canine Tissue Type Plasminogen Activator ELISA Kit |
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| MBS015661-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Canine Tissue Type Plasminogen Activator ELISA Kit |
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| MBS015661-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Canine Tissue Type Plasminogen Activator ELISA Kit |
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| MBS015661-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Canine Tissue Type Plasminogen Activator ELISA Kit |
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| MBS015661-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Canine Tissue type Plasminogen Activator ELISA Kit |
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| MBS741760-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 5685 |
Canine Tissue type Plasminogen Activator ELISA Kit |
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| MBS741760-48StripWells | MyBiosource | 48-Strip-Wells | EUR 485 |
Canine Tissue type Plasminogen Activator ELISA Kit |
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| MBS741760-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3020 |
Canine Tissue type Plasminogen Activator ELISA Kit |
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| MBS741760-96StripWells | MyBiosource | 96-Strip-Wells | EUR 690 |
*Canine Plasminogen Affinity Purified |
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| ICNPLGAP10MG | Innovative research | each | EUR 3248 |
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Description: *Canine Plasminogen Affinity Purified |
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*Canine Plasminogen Affinity Purified |
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| ICNPLGAP1MG | Innovative research | each | EUR 443 |
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Description: *Canine Plasminogen Affinity Purified |
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Canine Plasminogen Activator, Urokinase Receptor ELISA Kit |
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| MBS108772-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Canine Plasminogen Activator, Urokinase Receptor ELISA Kit |
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| MBS108772-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Canine Plasminogen Activator, Urokinase Receptor ELISA Kit |
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| MBS108772-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Canine Plasminogen Activator, Urokinase Receptor ELISA Kit |
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| MBS108772-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Cyno monkey plasminogen total antigen assay ELISA kit |
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| MBS136009-1Kit | MyBiosource | 1Kit | EUR 615 |
Cyno monkey plasminogen total antigen assay ELISA kit |
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| MBS136009-5Kits | MyBiosource | 5Kits | EUR 2175 |
Cyno monkey plasminogen total antigen assay ELISA kit |
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| MBS136009-5x5Kits | MyBiosource | 5x5Kits | EUR 10060 |
Canine Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit |
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| EIA06168Ca | Enlibio | each | EUR 520 |
Canine Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit |
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| MBS2603401-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3545 |
Canine Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit |
|||
| MBS2603401-48StripWells | MyBiosource | 48-Strip-Wells | EUR 310 |
Canine Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit |
|||
| MBS2603401-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1940 |
Canine Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit |
|||
| MBS2603401-96StripWells | MyBiosource | 96-Strip-Wells | EUR 475 |
Canine Plasminogen Activator Inhibitor 1(PAI1)ELISA Kit |
|||
| NSL1964Ca | Sunlong | 2059 wells | EUR 528 |
|
Description: Sandwich ELISA |
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Canine urokinase plasminogen activator(uPA)ELISA Kit |
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| EIA06499Ca | Enlibio | each | EUR 520 |
Canine urokinase plasminogen activator (uPA) ELISA Kit |
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| MBS2603084-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3545 |
Canine urokinase plasminogen activator (uPA) ELISA Kit |
|||
| MBS2603084-48StripWells | MyBiosource | 48-Strip-Wells | EUR 310 |
Canine urokinase plasminogen activator (uPA) ELISA Kit |
|||
| MBS2603084-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1940 |
Canine urokinase plasminogen activator (uPA) ELISA Kit |
|||
| MBS2603084-96StripWells | MyBiosource | 96-Strip-Wells | EUR 475 |
Canine Urokinase Plasminogen Activator(uPA)ELISA Kit |
|||
| NSL2014Ca | Sunlong | 2109 wells | EUR 528 |
|
Description: Sandwich ELISA |
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Canine urokinase plasminogen activator (uPA) ELISA Kit |
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| SL0041Ca | Sunlong | - | EUR 528 |
Canine Plasminogen activator inhibitor 1,PAI-1 ELISA KIT |
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| JOT-EKA0001Ca | Jotbody | 96 wells | EUR 380 |
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Description: Canine |
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Canine Plasminogen activator inhibitor 1,PAI-1 ELISA KIT |
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| EA0001Ca | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 475 |
Canine Plasminogen activator inhibitor 1, PAI-1 ELISA Kit |
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| MBS1609069-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 4295 |
Canine Plasminogen activator inhibitor 1, PAI-1 ELISA Kit |
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| MBS1609069-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2185 |
Canine Plasminogen activator inhibitor 1, PAI-1 ELISA Kit |
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| MBS1609069-96StripWells | MyBiosource | 96-Strip-Wells | EUR 520 |
Canine Plasminogen activator inhibitor 1, PAI-1 ELISA Kit |
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| MBS9305301-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Canine Plasminogen activator inhibitor 1, PAI-1 ELISA Kit |
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| MBS9305301-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Canine Plasminogen activator inhibitor 1, PAI-1 ELISA Kit |
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| MBS9305301-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Canine Plasminogen activator inhibitor 1, PAI-1 ELISA Kit |
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| MBS9305301-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Canine Plasminogen activator inhibitor 1,PAI-1 ELISA Kit |
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| YLA0044CA-48T | Shanghai YL Biotech | 48T | Ask for price |
Canine Plasminogen activator inhibitor 1,PAI-1 ELISA Kit |
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| YLA0044CA-96T | Shanghai YL Biotech | 96T | Ask for price |
Plasminogen Antibody |
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| C21629-100ul | Assay Biotech | 100μl | EUR 217 |
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Description: Plasminogen Rabbit Polyclonal Antibody |
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Plasminogen Antibody |
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| C21629-50ul | Assay Biotech | 50μl | EUR 143.5 |
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Description: Plasminogen Rabbit Polyclonal Antibody |
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Plasminogen (Plg) Magnetic Luminex Assay Kit |
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| LKU604009-96T | Biomatik Corporation | 96T | EUR 772.8 |
Plasminogen (Plg) Magnetic Luminex Assay Kit |
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| LKU604020-96T | Biomatik Corporation | 96T | EUR 995.9 |
Plasminogen (Plg) Magnetic Luminex Assay Kit |
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| LKU604030-96T | Biomatik Corporation | 96T | EUR 1101.7 |
Plasminogen (Plg) Magnetic Luminex Assay Kit |
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| LKU604036-96T | Biomatik Corporation | 96T | EUR 1101.7 |
SPARCL™ Plate (96-well) SPARCL™ Assay |
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| SP-PL | Life Diagnostics | 1 kit | EUR 100 |
Canine Urokinase type Plasminogen Activator Receptor ELISA kit |
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| E01A68165 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Operator efficiency was evaluated together with the analytical specificity and sensitivity (ASp and ASe) and diagnostic accuracy of the equipment, with parameters similar to diagnostic specificity and sensitivity (DSp and DSe) and optimistic and adverse predictive values (PPV and NPV), along with the repeatability. In accordance with the evaluated parameters, the diagnostic ELISA check was proven to be appropriate for validation and commercialization as a screening technique in human sera and a sound help for scientific diagnostics.