Chloramphenicol-activated electro-chemiluminescent behavior of BNQDs-Ru(phen)32+ system for ultra-sensitive sensing of chloramphenicol in pharmaceutical and milk samples

February 21, 2022 0 Comments

To improve the sensitivity for electro-chemiluminescent (ECL) detection of chloramphenicol (CAP), a common broad-spectrum antibiotic, boron nitride quantum dots (BNQDs) were prepared with excellent photoelectric property and low toxicity. After its structure and electrochemical property were investigated in detail, it was noted that the ECL signal of Ru(Phen)32+ could be strengthened by the proposed BNQDs, which was further activated by ten’s times in the presence of CAP. Under the optimized conditions, there was an excellent linear relationship between △ECL and lgcCAP in a wide linear range from 1.0×10-10 to 1.0×10-6 mol/L CAP.
The detection limit was super-low to be 3.3×10-11 mol/L (S/N=3). When applied for CAP detection in real pharmaceutical and food samples, the recoveries were between 97.8 and 105.7 % with R.S.D. less than 3.3%. A possible CAP-activated ECL mechanism of BNQDs-Ru(phen)32+ was also proposed. This work will offer a great potential for efficient monitoring of CAP pollution and clinical diagnosing of CAP-related diseases in future.

Chloramphenicol-activated electro-chemiluminescent behavior of BNQDs-Ru(phen)32+ system for ultra-sensitive sensing of chloramphenicol in pharmaceutical and milk samples

To improve the sensitivity for electro-chemiluminescent (ECL) detection of chloramphenicol (CAP), a common broad-spectrum antibiotic, boron nitride quantum dots (BNQDs) were prepared with excellent photoelectric property and low toxicity. After its structure and electrochemical property were investigated in detail, it was noted that the ECL signal of Ru(Phen)32+ could be strengthened by the proposed BNQDs, which was further activated by ten’s times in the presence of CAP. Under the optimized conditions, there was an excellent linear relationship between △ECL and lgcCAP in a wide linear range from 1.0×10-10 to 1.0×10-6 mol/L CAP.
The detection limit was super-low to be 3.3×10-11 mol/L (S/N=3). When applied for CAP detection in real pharmaceutical and food samples, the recoveries were between 97.8 and 105.7 % with R.S.D. less than 3.3%. A possible CAP-activated ECL mechanism of BNQDs-Ru(phen)32+ was also proposed. This work will offer a great potential for efficient monitoring of CAP pollution and clinical diagnosing of CAP-related diseases in future.
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Highly Sensitive Chemiluminescent Immunoassay of Mycotoxins Using ZIF-8-Derived Yolk-Shell Co Single-Atom Site Catalysts as Superior Fenton-like Probes

  • Superior to traditional nanoscale catalysts, single-atom site catalysts (SASCs) show such merits as maximal catalysis efficiency and outstanding catalytic activity for the construction of analytical methodological platforms. Hereby, an in situ etching strategy was designed to prepare yolk-shell Co SASCs derived from ZIF-8@SiO2 nanoparticles. On the basis of direct chemical interactions between precursors and supports, the Co element with isolated atomic dispersion was anchored on ZIF-8@SiO2 nanoparticles.
  • The Co SASCs possess high Fenton-like activity and thus can catalyze the decomposition of H2O2 to produce massive superoxide radical anions instead of singlet oxygen and hydroxyl radicals. With the activity for producing superoxide radical anion, Co SASCs can greatly improve the chemiluminescent (CL) response of a luminol system by 3133.7 times. Furthermore, the SASCs with active sites of Co-O5 moieties were utilized as the CL probes for establishment of an immunoassay method for sensitive detection of mycotoxins by adopting aflatoxin B1 as a mode analyte.
  • The quantitation range is 10-1000 pg/mL, and the limit of detection is 0.44 pg/mL (3σ) for aflatoxin B1. The proof-of-principle work elucidates the practicability of direct chemical interactions between precursors and supports for forming SASCs with ultrahigh CL response, which can be extended to the exploitation of more sorts of SASCs for tracing biological binding events.

Tri-iodide and vanadium chloride based chemiluminescent methods for quantification of nitrogen oxides

Nitric Oxide (NO) is an important signaling molecule that plays roles in controlling vascular tone, hemostasis, host defense, and many other physiological functions. Low NO bioavailability contributes to pathology and NO administration has therapeutic potential in a variety of diseases. Thus, accurate measurements of NO bioavailability and reactivity are critical.
Due to its short lifetime in vivo and many in vitro conditions, NO bioavailability and reactivity are often best determined by measuring NO congeners and metabolites that are more stable. Chemiluminescence-based detection of NO following chemical reduction of these compounds using the tri-iodide and vanadium chloride methods have been widely used in a variety of clinical and laboratory studies. In this review, we describe these methods used to detect nitrite, nitrate, nitrosothiols and other species and discuss limitations and proper controls.

Chemiluminescent Probes Based on 1,2-dioxetane Structures For Bioimaging

Chemiluminescent probes based on 1,2-dioxetane scaffold are one of the most sensitive imaging modalities for detecting disease-related biomarkers and can obtain more accurate biological information in cells and in vivo . Due to the elimination of external light excitation, the background autofluorescence problem in fluorescence technology can be effectively avoided, providing ultra-high sensitivity and signal-to-noise ratio for various applications.
In this minireview, we highlight a comprehensive but concise overview of activatable 1,2-dioetxane-based chemiluminescent probes by reporting significant advances in accurate detection and bioimaging. The design principles and applications for reactive species, enzymes, and other disease-related biomarkers are systematically discussed and summarized. The challenges and potential prospects of chemiluminescent probes are also discussed to further promote the development of new chemiluminescence methods for biological analysis and diagnosis.

FTO Chemiluminescent Assay Kit

79344 BPS Bioscience 96 rxns. 765 EUR

UTX Chemiluminescent Assay Kit

50615 BPS Bioscience 96 rxns. 715 EUR

G9a Chemiluminescent Assay Kit

52001L BPS Bioscience 96 rxns. 750 EUR

NSD3 Chemiluminescent Assay Kit

79358 BPS Bioscience 384 rxns. 1950 EUR

NSD2 Chemiluminescent Assay Kit

79359 BPS Bioscience 384 rxns. 1100 EUR

P300 Chemiluminescent Assay Kit

79705 BPS Bioscience 96 rxns. 465 EUR

EZH1 Chemiluminescent Assay Kit

52990 BPS Bioscience 384 rxns. 4120 EUR

NSD2 Chemiluminescent Assay Kit

53009 BPS Bioscience 96 rxns. 750 EUR

NSD3 Chemiluminescent Assay Kit

53012 BPS Bioscience 96 rxns. 750 EUR

GCN5 Chemiluminescent Assay Kit

50079L BPS Bioscience 96 rxns. 465 EUR

TET1 Chemiluminescent Assay Kit

50651 BPS Bioscience 96 rxns. 725 EUR

TET2 Chemiluminescent Assay Kit

50652 BPS Bioscience 96 rxns. 760 EUR

EZH2 Chemiluminescent Assay Kit

52009L BPS Bioscience 96 rxns. 1550 EUR

EZH1 Chemiluminescent Assay Kit

52079 BPS Bioscience 96 rxns. 1550 EUR

EZH2 Chemiluminescent Assay Kit

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cAMP ELISA Kit (Chemiluminescent)

STA-501 Cell Biolabs 96 assays 706.8 EUR

cAMP ELISA Kit (Chemiluminescent)

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cGMP ELISA Kit (Chemiluminescent)

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cGMP ELISA Kit (Chemiluminescent)

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PRDM9 Chemiluminescent Assay Kit

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PARP7 Chemiluminescent Assay Kit

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PARP7 Chemiluminescent Assay Kit

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SMYD4 Chemiluminescent Assay Kit

53013 BPS Bioscience 96 rxns. 725 EUR

PRMT5 Chemiluminescent Assay Kit

52002L BPS Bioscience 96 rxns. 865 EUR

PRMT1 Chemiluminescent Assay Kit

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PRMT3 Chemiluminescent Assay Kit

52005L BPS Bioscience 96 rxns. 750 EUR

PRMT4 Chemiluminescent Assay Kit

52041L BPS Bioscience 96 rxns. 750 EUR

DNMT1 Chemiluminescent Assay Kit

52050L BPS Bioscience 96 rxns. 865 EUR

SETD2 Chemiluminescent Assay Kit

52060 BPS Bioscience 96 rxns. 750 EUR

PRMT8 Chemiluminescent Assay Kit

52062 BPS Bioscience 96 rxns. 750 EUR

Comparative evaluation of chemiluminescent immunoassay and enzyme-linked immunosorbent assays for the diagnosis of West Nile Virus infections

In August 2020, a new West Nile Virus (WNV) outbreak affected 71 people with meningoencephalitis in Andalusia (Spain). Samples from these individuals were received by our laboratory, a regional Virus Referral Centre. The aim of this study was to compare the agreement, sensitivity, and specificity of findings between the WNV VIRCLIA IgG and IgM assay (Vircell, Spain) and the WNV ELISA IgM and IgG assay (Euroimmun, Germany) and to compare the performance of WNV VIRCLIA IgM and Euroimmun ELISA for cerebrospinal fluid (CSF) diagnosis. The study included 24 CSF samples (paired with serum samples) and 247 serum samples from 217 patients with suspected WNV infection (1 or 2 per patient).
The agreement between ELISA and CLIA tests for IgM and Ig G detection in serum was 93% (kappa index= 0.85) and 96% (kappa index = 0.89), respectively. Sensitivity values of ELISA and CLIA tests for IgM in serum samples were 96.7% and 98.9%, respectively, and specificity values were 96.4% and 95.4%, respectively. Sensitivity values of ELISA and CLIA test for IgG in serum samples were 91.1% and 97%, respectively, and specificity values were 100% and 98.8%, respectively. Results obtained with ELISA and CLIA tests in CSF samples showed 75% agreement between them (kappa index=0.51). According to these findings, the WNV VIRCLIA IgM and IgG monotest offers an accurate qualitative detection of WNV in serum and CSF specimens.

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