The New and the Old: Platform Cross-Validation of Immunoaffinity MASS Spectrometry versus ELISA for PromarkerD, a Predictive Test for Diabetic Kidney Disease

August 23, 2021 Taylor 0 Comments

PromarkerD is a proteomics derived take a look at for predicting diabetic kidney illness that measures the concentrations of three plasma protein biomarkers, APOA4, CD5L and IBP3. Antibodies in opposition to these proteins had been developed and utilized to a multiplexed immunoaffinity seize mass spectrometry assay. In parallel, and facilitating present scientific laboratory workflows, a typical ELISA was additionally developed to measure every protein.

The efficiency traits of the 2 know-how platforms had been in contrast utilizing a cohort of 100 samples, with PromarkerD take a look at scores demonstrating a excessive correlation (R = 0.97). These applied sciences illustrate the potential for big scale, excessive throughput scientific purposes of proteomics now and into the longer term.

Correlation of ELISA methodology with three different automated serological exams for the detection of anti-SARS-CoV-2 antibodies

Public well being emergency of SARS-CoV-2 has facilitated diagnostic testing as a associated medical countermeasure in opposition to COVID-19 outbreak. Quite a few serologic antibody exams have grow to be obtainable by an expedited federal emergency use solely course of. This paper highlights the analytical attribute of an ELISA based mostly assay by AnshLabs and three random entry immunoassay (RAIA) by DiaSorin, Roche, and Abbott which were accredited for emergency use authorization (EUA), at a tertiary educational middle in a low disease-prevalence space.

The AnshLabs gave increased estimates of sero-prevalence, over the three RAIA strategies. For constructive outcomes, AnshLabs had 93.3% and 100% settlement with DiaSorin or Abbott and Roche respectively. For destructive outcomes, AnshLabs had 74.3% and 78.3% settlement with DiaSorin and Roche or Abbott respectively. All discrepant samples that had been constructive by AnshLabs and destructive by RAIA examined constructive by all-in-one step SARS-CoV-2 Complete (COV2T) assay carried out on the automated Siemens Advia Centaur XPT analyzer. None of those strategies, nonetheless, are helpful in early prognosis of SARS-CoV-2.

Improvement and Validation of a Fast Lateral Movement E1/E2-Antigen Take a look at and ELISA in Sufferers Contaminated with Rising Asian Pressure of Chikungunya Virus within the Americas

Since its 2013 emergence within the Americas, Chikungunya virus (CHIKV) has posed a critical risk to public well being. Early and correct prognosis of the illness, although at the moment missing in clinics, is integral to allow well timed care and epidemiological response. We developed a twin detection system: a CHIKV antigen E1/E2-based enzyme-linked immunosorbent assay (ELISA) and a lateral move take a look at utilizing high-affinity anti-CHIKV antibodies.

The ELISA was validated with 100 PCR-tested acute Chikungunya fever samples from Honduras. The assay had an total sensitivity and specificity of 51% and 96.67%, respectively, with accuracy reaching 95.45% sensitivity and 92.03% specificity at a cycle threshold (Ct) cutoff of 22. Because the Ct worth decreased from 35 to 22, the ELISA sensitivity elevated. We then developed and validated two lateral move exams utilizing impartial antibody pairs.

The sensitivity and specificity reached 100% for each lateral move exams utilizing 39 samples from Colombia and Honduras at Ct cutoffs of 20 and 27, respectively. For each lateral move exams, sensitivity decreased because the Ct elevated after 27. As a result of CHIKV E1/E2 are uncovered within the virion surfaces in serum throughout the acute an infection section, these delicate and particular assays exhibit alternatives for early detection of this rising human pathogen.

Investigation of Anti-SARS-CoV-2 IgG and IgM Antibodies within the Sufferers with COVID-19 by Three Totally different ELISA Take a look at Kits

We examined anti-SARS-CoV-2 IgG and IgM antibodies in 45 serum samples from 26 sufferers with COVID-19, who had been admitted in our hospital through the use of three totally different ELISA kits. All sufferers had pneumonia at admission, and seven sufferers required mechanical ventilator help and grouped in extreme case. Anti-SARS-CoV-2 IgG and IgM antibodies turned to be partially constructive between the sixth and 10th days, greater than 84% constructive between the 11th and 15th days, and 100% after the 16th day.

One ELISA package revealed poorer sensitivity for anti-SARS-CoV-2 IgM antibody. Detrimental conversion of IgM antibody was not noticed within the 30th day in our cohort. All three ELISA kits confirmed no false constructive response for destructive serum samples. Between extreme and reasonable circumstances, there was no important distinction within the traits of anti-SARS-CoV-2 IgG and IgM antibody.

Promising utility of the SsCBF ELISA take a look at to observe the therapeutic response of feline sporotrichosis attributable to Sporothrix brasiliensis from Brazilian epidemics

Sporotrichosis zoonotic transmission by cats has obtained hyperendemic magnitude in Rio de Janeiro, Brazil. Atypical circumstances, relapses, and reinfections in addition to decreased diagnostic sensitivity of standard strategies have been reported. Beforehand, the anti-SsCBF enzyme-linked immunosorbent assay (ELISA) take a look at was proven to be helpful as a diagnostic instrument for human sporotrichosis. Efficient prognosis and remedy are crucial to interrupt the chain of transmission of this main pathogen in Brazilian Public Well being.

To judge its applicability for feline sporotrichosis prognosis and/or therapeutic follow-up, 15 home cats from Rio de Janeiro had been clinically and laboratory monitored by cytopathology, tradition, Sporothrix genotyping, and anti-SsCBF IgG ranges. Subsequently, animals had been divided into passable and non-satisfactory therapeutic responders.

96 well filter plate (960ul each well)
SD5006	Bio Basic	12UNIT	EUR 115.85

Black 96 Well Plate, 1EA
X025-1EA	Arbor Assays	1EA	EUR 19

96 Deep Well Plate Square Well V
PCR0246	Scientific Laboratory Supplies	PK50	EUR 322.8

Adapter for 96 well plate
3510147	Atto	1unit	EUR 6697.2

UNIFILTER PLATE 96 WELL 800 UL/WELL
WHA77002803	Scientific Laboratory Supplies	PK25	EUR 979.2

Plate 96 Well Immulon 1 FB
N3355	Scientific Laboratory Supplies	PK50	EUR 207.6

Brand 96 Well Deep Well Plate-Stackable
BR701354-24EA	Scientific Laboratory Supplies	PK24	EUR 163.2

96 well PCR plate ns
AXY2164	Scientific Laboratory Supplies	PK50	EUR 238.8

MULTIWELL PLATE, POLYSTYRENE 96-WELL U-B
M2186-100EA	Scientific Laboratory Supplies	PK100	EUR 131.87

Aligned Nanofibers 96 Well Plate
3D00012-96	Neuromics	700 nm-PCLs	EUR 132

Streptavidin coated 96-well Plate
6523-5	Biovision	each	EUR 392.4

96-Well ROCK Activity Assay Kit
STA-416	Cell Biolabs	96 assays	EUR 1027.2
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit
STA-416-5	Cell Biolabs	5 x 96 assays	EUR 4228.8
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE-96001	Favorgen	1 prep	EUR 175.2

Plate 96 Well Immulon II
3455	Scientific Laboratory Supplies	PK50	EUR 283.2

OxiSelect 96-Well Comet Assay Kit
STA-355	Cell Biolabs	96 assays	EUR 512.4
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit
STA-355-5	Cell Biolabs	5 x 96 assays	EUR 1959.6
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

DEEP WELL PLATE 96 WELL 2.0 ML STERIL
AXYPDW20CS	Scientific Laboratory Supplies	PK50	EUR 240

96-Well Plate Viral DNA Miniprep Kit
VT92032	Bio Basic	2XPlates, 192prep	EUR 495.86

96 Well Skirted PCR Plate
PCR1236	Scientific Laboratory Supplies	PK50	EUR 385.2

96-Well Plate Plasmid DNA Miniprep Kit
BS415	Bio Basic	5XPlates, 480prep	EUR 300.37

96-Well Plate Plasmid DNA Miniprep Kit
BS4152	Bio Basic	2XPlates, 192prep	EUR 186.19

96 Well Semi Skirted Plate
PCR1216	Scientific Laboratory Supplies	PK50	EUR 204

96-Well Plate DNA Cleanup Miniprep Kit
BS369	Bio Basic	5XPlates, 480prep	EUR 300.37

96-Well Plate DNA Cleanup Miniprep Kit
BS3692	Bio Basic	2xPlates, 192prep	EUR 186.19

96 Deep Well Plate Round Wells
PCR0242	Scientific Laboratory Supplies	PK50	EUR 292.8

Nunc Lid for 96 well plate
264122	Scientific Laboratory Supplies	PK50	EUR 127.2

96 WELL PCR SEGMENTED PLATE
PCR-96-SG-C	CORNING	10/pk	EUR 174
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 Deep Well Plate Square Wells
PCR0240	Scientific Laboratory Supplies	PK100	EUR 336

96 Deep Well Plate Square WellU
PCR0256	Scientific Laboratory Supplies	PK50	EUR 232.8

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY
P-DW-12-HC	CORNING	5/pk	EUR 182.4
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Tissue Culture Plate, 96 Well
TCP20-96	Bio Basic	1 UNIT	EUR 64.18

Clear Coated 96 Well Plate, 1EA
C107-1EA	Arbor Assays	1EA	EUR 162

96-Well Plate PCR Products Purification Kit
BS365	Bio Basic	5XPlates, 480prep	EUR 300.37

96-Well Plate PCR Products Purification Kit
BS3652	Bio Basic	2xPlates, 192prep	EUR 186.19

96 Well PCR Plate Clear Wells
PCR0972	Scientific Laboratory Supplies	PK50	EUR 258

96-Well Protein Folding Plate
PFS096	ProFoldin	kit	EUR 347.84
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins.

Random Access 96 Well Plate Skir
PCR1412	Scientific Laboratory Supplies	PK50	EUR 301.2

96 WELL PLATE; SQUARE; 2ML; PP; 1/PK
60180-P403	Scientific Laboratory Supplies	EACH	EUR 19.2

Falcon Microtest Black 96 Well Plate
353376	Scientific Laboratory Supplies	PK32	EUR 217.2

96 Well ABI Fast Plate Pcr
PCR1200	Scientific Laboratory Supplies	PK50	EUR 258

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTA55-5P	Alpha Diagnostics	1 PK	EUR 315.6

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTG65-5P	Alpha Diagnostics	1 PK	EUR 315.6

96 Deep Well Plate Round Wells U
PCR0228	Scientific Laboratory Supplies	PK100	EUR 217.2

96 Deep Well Plate Round Wells V
PCR0230	Scientific Laboratory Supplies	PK100	EUR 217.2

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031	Bio Basic	2Plates, 192prep	EUR 210.85

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032	Bio Basic	5Plates, 480prep	EUR 389.9

96 Well Plate Yeast Genomic DNA Miniprep Kit
BS8357	Bio Basic	2XPlates, 192prep	EUR 234.6

Nunc Lid for 96 Well Plate SH
263339	Scientific Laboratory Supplies	PK100	EUR 112.8

2 x Clear 96 well Plate, 2EA
X003-2EA	Arbor Assays	2EA	EUR 13

5 x Clear 96 well Plate, 5EA
X003-5EA	Arbor Assays	5EA	EUR 25

2 x Clear 96 well Plate, 2EA
X018-2EA	Arbor Assays	2EA	EUR 13

4 x Clear 96 Well Plate, 4EA
X018-4EA	Arbor Assays	4EA	EUR 31

96-Well Plate Plant Genomic DNA Miniprep Kit
BS8361	Bio Basic	2XPlates, 192prep	EUR 234.6

PCR Plate Non Skirted 96 Well
MOL2364	Scientific Laboratory Supplies	PK50	EUR 309.6

96 Well Roche Style Pcr Plate
PCR1214	Scientific Laboratory Supplies	PK50	EUR 294

96 Well Roche Style PCR Plate
PCR1218	Scientific Laboratory Supplies	PK50	EUR 271.2

Polystyrene-bottomed 96-well plate + lid (ELISPOT)
CT365	U-CyTech	5	EUR 93.6

PCR Plate Semi Skirted 96 Well
MOL2366	Scientific Laboratory Supplies	PK50	EUR 301.2

96 Well Roche Style Qpcr Plate
PCR1210	Scientific Laboratory Supplies	PK50	EUR 283.2

96 well filter plate (no filter)
SD5006-EMPTY	Bio Basic	12UNIT	EUR 104.89

96 Well Plate RNA Cleanup and Concentration Kit
BRC1251	Bio Basic	2xPlates, 192prep	EUR 300.37

96-Well Plate Animal Genomic DNA Miniprep Kit
BS437	Bio Basic	5XPlates, 480prep	EUR 435.58

96-Well Plate Animal Genomic DNA Miniprep Kit
BS4372	Bio Basic	2XPlates, 192prep	EUR 234.6

96 Well Skirted Plate+ Q-Stick
PCR1238	Scientific Laboratory Supplies	PK50	EUR 277.2

Benchmark Beadblaster 96 Adapter for 96 Well Plate
HOM3208	Scientific Laboratory Supplies	PK2	EUR 1710

Black Half Area 96 Well Plate, 1EA
X023-1EA	Arbor Assays	1EA	EUR 25

96 Well Solid Bottom Plate Black
PCR0354	Scientific Laboratory Supplies	PK100	EUR 422.4

96 Well Solid Bottom Plate White
PCR0360	Scientific Laboratory Supplies	PK100	EUR 388.8

96 Well PCR Plate Divisible In12
PCR1064	Scientific Laboratory Supplies	PK50	EUR 174

96 Well Semi-Skirted PCR Plate
PCR1106	Scientific Laboratory Supplies	PK50	EUR 368.4

S100 96-well PCR Plate Adapter
E5391070026	Scientific Laboratory Supplies	EACH	EUR 379.2

S200 96-well PCR Plate Adapter
E5392070038	Scientific Laboratory Supplies	EACH	EUR 379.2

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1292	Bio Basic	2XPlates, 192prep	EUR 210.85

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1295	Bio Basic	5XPlates, 480prep	EUR 389.9

Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTAG75-5P	Alpha Diagnostics	1 PK	EUR 343.2

96-well Checkpoint Kinase Activity Assay Kit
STA-414	Cell Biolabs	96 assays	EUR 908.4
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit
STA-414-5	Cell Biolabs	5 x 96 assays	EUR 3672
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96 well DNA plate with membrane (960ul each well)
SD5007	Bio Basic	12UNIT	EUR 291.24

96 well RNA plate with membrane (960ul each well)
SD5009	Bio Basic	12UNIT	EUR 313.16

2'3'-Cyclic GAMP ELISA Kit, (384-well Plate)
K067-H1D	Arbor Assays	1x384 well plate	EUR 1557

Benchmark Beadblaster 96 Eva Cap Mat for 96 Well Deep Well Plate
HOM3232	Scientific Laboratory Supplies	EACH	EUR 121.2

96 WELL PLATE; SQUARE; U BASE; 1.6ML; PP; 96/PK
60180-P155	Scientific Laboratory Supplies	PK96	EUR 561.6

PCR Plate 96 Well No Skirt Nat
LW2200	Scientific Laboratory Supplies	PK100	EUR 534

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)
CT366	U-CyTech	5	EUR 268.8

MULTIWELL PLATE POLYSTYRENE 96-WELL FLA
M2936-100EA	Scientific Laboratory Supplies	PK100	EUR 131.87

Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)
M-R-1008649	MiTeGen	98 X 1.7 ml	EUR 316
Description: Wizard Cryo 1 and 2 96 Well Matrix Block Plate (96 conditions 1.7 mL volumes in a 96-Well block plate)

96 WELL CLEAR PCR PLATE FOR ABI
PCR-96-AB-C	CORNING	10/pk	EUR 200.4
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92028	Bio Basic	2XPlates, 192prep	EUR 254.7

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92029	Bio Basic	5XPlates, 480prep	EUR 437.4

Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
M-R-1008647	MiTeGen	96 X 1.7 ml	EUR 316
Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

96 Well PCR Plate Divisible In 8
PCR1060	Scientific Laboratory Supplies	PK50	EUR 174

96 Well PCR Plate Divisible In 8
PCR1062	Scientific Laboratory Supplies	PK50	EUR 187.2

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)
EXOEL-96P-1	SBI	10 Plates	EUR 255.6

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS585	Bio Basic	5XPlates, 480prep	EUR 585.38

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS5852	Bio Basic	2XPlates, 192prep	EUR 300.37

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED
P-DW-12-HC-S	CORNING	5/pk	EUR 248.4
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Thermo Fast 96 Well PCR Plate - PK25
AB0800	Scientific Laboratory Supplies	PK25	EUR 254.4

Block for 96 well plate, WSC-2620/30
4002659	Atto	1unit	EUR 458.4
Description: Bl ock for WSC-2620 / 2630

UNIFILTER PLATE 96 WELL 800 UL/PER WEL
WHA77002801	Scientific Laboratory Supplies	EACH	EUR 976.8

CORNING® BIOCOAT™ GELATIN 96 WELL CLEAR FLAT BOTTOM ASSAY PLATE, WITH LID, NONSTERILE, 5/PACK, 50/CASE
356689	CORNING	5/pk	EUR 800.4
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

96 Well Lung Tumor Genomic DNA Plate
D8235152-1	Biochain	1 plate	EUR 328

Radius 96-Well Cell Migration Assay
CBA-126	Cell Biolabs	96 assays	EUR 686.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay
CBA-126-5	Cell Biolabs	5 x 96 assays	EUR 2697.6
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

OxiSelect 96-Well Comet Assay Slide
STA-356	Cell Biolabs	1 slide	EUR 219.6
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide
STA-356-5	Cell Biolabs	5 slides	EUR 622.8
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

96 Well Colon Tumor Genomic DNA Plate
D8235090-1	Biochain	1 plate	EUR 328

Modular In Situ-1 96 Well Crystallization Plate
ML-MIS-FP3D	MiTeGen	1 PLATE	EUR 392
Description: Modular In Situ-1 96 Well Crystallization Plate

96 Well Breast Tumor Genomic DNA Plate
D8235086-1	Biochain	1 plate	EUR 328

96 Well Impact MicroBatch Plate, 80/CSE
M-673101	MiTeGen	80 PLATES	EUR 808
Description: 96 Well Impact MicroBatch Plate, 80/CSE

96-well PCR Plate Non-Skirted (White)
AB-0600W	Scientific Laboratory Supplies	PK25	EUR 256.8

96 Well Stomach Tumor Genomic DNA Plate
D8235248-1	Biochain	1 plate	EUR 328

384 Well Deep Well Plate V
PCR0316	Scientific Laboratory Supplies	PK100	EUR 512.4

Mouse anti-ACTH Clear Coated 96 Well Plate
C271-1EA	Arbor Assays	1EA	EUR 150

CORNING® BIOCOAT™ T-CELL ACTIVATION CONTROL 96 WELL FLAT BOTTOM ASSAY PLATE, INDIVIDUALLY WRAPPED, 5/CASE
354730	CORNING	5/pk	EUR 102
Description: Advanced Cells - DL; BioCoat 96 Well Clear - DL

96-well PCR Plate Non-Skirted natural
AB-0600	Scientific Laboratory Supplies	PK25	EUR 181.2

Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA
X011-1EA	Arbor Assays	1EA	EUR 48

6 Well Plate BD
354721	Scientific Laboratory Supplies	PK5	EUR 90

2 x Black Half Area 96 Well Plate, 2EA
X037-2EA	Arbor Assays	2EA	EUR 37

96-well PCR Plate (Segmented Semi-Skirted)
AB-0900	Scientific Laboratory Supplies	PK25	EUR 184.8

PCR Plate 384 Well
MOL2370	Scientific Laboratory Supplies	PK50	EUR 543.6

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)
B814152-0002	Bio Basic	2XPlates, 2XPlates	EUR 186.19

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)
B814152-0005	Bio Basic	5XPlates, 5XPlates	EUR 300.37

Clear 384 Well Plate
X105-1EA	Arbor Assays	1EA	EUR 73

Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
M-R-1008648	MiTeGen	97 X 1.7 ml	EUR 316
Description: Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)

CytoSelect 96-well Cell Transformation Assay
CBA-130	Cell Biolabs	96 assays	EUR 866.4
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay
CBA-130-5	Cell Biolabs	5 x 96 assays	EUR 3463.2
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

SuperPlate 96-well PCR Plate Semi-Skirted
AB-2400	Scientific Laboratory Supplies	PK25	EUR 235.2

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224	Cell Biolabs	96 assays	EUR 866.4
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224-5	Cell Biolabs	5 x 96 assays	EUR 3525.6
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

Benchmark Beadblaster 96 96 Well Plate Prefilled with 4mm Beads
HOM3228	Scientific Laboratory Supplies	PK10	EUR 2634

Adapter for 96-well PCR plate 2 pcs.
E5825711009	Scientific Laboratory Supplies	PK2	EUR 158.4

DB 6.3 Double block; 1 x 96 well PCR plate
BLO6082	Scientific Laboratory Supplies	EACH	EUR 394.8

Averages of antibody serum ranges obtained for prognosis (first session) in contrast with the degrees discovered after follow-up (final session) had been considerably totally different in each teams (p = 0.0002 and p = 0.038, respectively). We conclude that the SsCBF ELISA take a look at can predict feline sporotrichosis therapeutic responses even for animals with distinct scientific evolutions.